Amoxicillin USP Assay by HPLC – AppNote
April 23, 2021
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Date: 23-APRIL-2021 Last Updated: 31-AUGUST-2026
Alternate L1 Column used for the USP Assay Method for Amoxicillin
This USP Amoxicillin Assay is performed and a five injection overlay shows Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values less than 0.3%. This demonstrates a good alternate Column for your Amoxicillin USP Method.
Peak: Amoxicillin
Met hod Conditions:Column: Cogent Hyper Similars C18™, 5 µm, 150Å
Catalog No.: 62518-25P
Dimensions: 4.6 x 250 mm
Mobile Phase: 96:4 Buffer / Acetonitrile
-- Buffer: 6.8 g / L Monobasic Potassium Phosphate ( adjusted with 45% Potassium Hydroxide to a pH of 5.0 +/- 0.1)
Injection vol.: 10 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 230 nm
Sample Preparation: Amoxicillin 1.2 mg / mL in Buffer
Note 1: Amoxicillin is a semisynthetic antibiotic and analog of ampicillin, with a broad spectrum of bactericidal activity against many Gram-positive and Gram-negative microorganisms.
Note 2: The Cogent Hyper Similars C18™ performs very similarly to the Thermo Fisher Hypersil® C18.
