Dexamethasone and Prednisone Assay by HPLC- AppNote
November 7, 2020
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Analysis of Corticosteroids
This simple isocratic Method demonstrates good separation of two similar synthetic Glucocorticoids . This analysis for corticosteroids may serve well for quantitation in pharmaceutical preparations. This Method would easily translate to LCMS.
Column: Cogent Bidentate C18™, 4 µm, 100Å
Catalog No.: 40018-75
Dimensions: 4.6 x 7 5mm
Mobile Phase: (70:30) DI Water with 0.1% Formic Acid / Acetonitrile
Injection vol.: 1 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 254 nm
Sample Preparation: Prednisone and Dexamethasone 0.1 mg / mL in Methanol.
Notes: Prednisolone and Dexamethasone are synthetic Glucocorticoids, a class of steroid hormones. These are produced by the adrenal gland and are known for anti-inflammatory and immunosuppressive actions
This simple isocratic Method demonstrates good separation of two similar synthetic Glucocorticoids . This analysis for corticosteroids may serve well for quantitation in pharmaceutical preparations. This Method would easily translate to LCMS.
Peak:
1. Prednisone
2. Dexamethasone
Column: Cogent Bidentate C18™, 4 µm, 100Å
Catalog No.: 40018-75
Dimensions: 4.6 x 7 5mm
Mobile Phase: (70:30) DI Water with 0.1% Formic Acid / Acetonitrile
Injection vol.: 1 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 254 nm
Sample Preparation: Prednisone and Dexamethasone 0.1 mg / mL in Methanol.
Notes: Prednisolone and Dexamethasone are synthetic Glucocorticoids, a class of steroid hormones. These are produced by the adrenal gland and are known for anti-inflammatory and immunosuppressive actions