Fructose and Glucose with HPLC & ELSD - AppNote
December 13, 2016
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Date:
10-OCTOBER-2025
Last Updated: 10-OCTOBER-2025
The Cogent Amide Column, combined with a TEA-based mobile phase, offers excellent separation performance, and the method is well-suited for routine sugar analysis in beverages.
The Cogent Amide Column, combined with a TEA-based mobile phase, offers excellent separation performance, and the method is well-suited for routine sugar analysis in beverages.
PEAKS:
1. D-Fructose
2. D-Glucose
Method Conditions
Column: Cogent Amide™, 4μm, 100Å
Catalog No.:
40036-10D
Dimensions: 4.6 x 100 mm
Mobile Phase:
10% DI Water / 90% Acetonitrile (v/v),
Injection vol.: 3μL
Flow rate: 1.0 mL/minute
Detection:
ELSD (Gain 8, Temp 50C, Nitrogen: 3.5lb)
Sample:
D-Fructose and D-Glucose reference standards (1 mg/mL) in diluent of 50% Acetonitrile / 50% DI Water / 0.1% TEA (v/v)