Guanidine Analyzed with HPLC- AppNote
February 21, 2024
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Date: 21-FEBRUARY-2024 Last Updated: 7-SEPTEMBER-2026
A Straightforward HPLC-UV method for GuanidineA simple Method was developed for the analysis of Guanidine, a highly basic (pKa 12.5), and polar compound. Pre-column derivatization was not used in this method, but if higher sensitivity is needed for lower concentrations, ELSD could be employed.
Peaks: 1. Impurity, 2. Guanidine
Method ConditionsColumn: Cogent Diamond Hydride™, 4 µm, 100 Å
Catalog No.: 70000-10P
Dimensions: 4.6 mm x 100 mm
Mobile Phase: 50% DI Water / 50% Acetonitrile / 0.1% Formic Acid
Injection vol.: 1 µL
Flow rate: 1.0 mL/minute
Detection: UV @195 nm
Sample Preparation: 1.7 mg/mL Guanidine in Mobile phase
Note: This method could be used for clearance testing of guanidine in biopharmaceutical products.
