Semaglutide Peptide Analyzed with HPLC - AppNote
April 23, 2021
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Semaglutide, a Human Glucagon-like Peptide
This simple isocratic Method demonstrates good Retention of a Hydrophilic Peptide where Retention is increased in Aqueous conditions.
Column: Cogent Diamond Hydride™, 4μm, 100Å.
Catalog No .: 70000-75
Dimensions: 4.6 x 75 mm
Mobile Phase: 60:30 DI Water with 0.1% Formic Acid / Acetonitrile.
Injection vol .: 1 µL
Flow rate: 1.0 mL / minute.
Detection: UV @ 254 nm.
Sample Preparation: 0.01 mg / mL Semaglutide in 50:50 Acetonitrile / DI Water.
Notes: Semaglutide is a glucagon-like peptide-1 receptor agonist. It increases the production of insulin, a hormone that lowers the blood sugar level. It also appears to enhance growth of β cells in the pancreas, which are the sites of insulin production.
This simple isocratic Method demonstrates good Retention of a Hydrophilic Peptide where Retention is increased in Aqueous conditions.
Peak:
Semaglutide
Column: Cogent Diamond Hydride™, 4μm, 100Å.
Catalog No .: 70000-75
Dimensions: 4.6 x 75 mm
Mobile Phase: 60:30 DI Water with 0.1% Formic Acid / Acetonitrile.
Injection vol .: 1 µL
Flow rate: 1.0 mL / minute.
Detection: UV @ 254 nm.
Sample Preparation: 0.01 mg / mL Semaglutide in 50:50 Acetonitrile / DI Water.
Notes: Semaglutide is a glucagon-like peptide-1 receptor agonist. It increases the production of insulin, a hormone that lowers the blood sugar level. It also appears to enhance growth of β cells in the pancreas, which are the sites of insulin production.