Metabolomics Development Kit, 2x50. 1 Cogent Diamond Hydride™ & 1 Cogent Bidentate C18™. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 43000‑05P‑2 Metabolomics Development Kit offers an economical, application‑focused pairing of two widely used TYPE‑C™ silica hydride HPLC columns for LC‑MS‑based metabolomics and related small‑molecule workflows. This kit includes one Cogent Diamond Hydride™ HPLC Column and one Cogent Bidentate C18™ HPLC Column, enabling direct comparison of HILIC‑style and reversed‑phase separations using matched column dimensions.
Both columns are manufactured with Cogent TYPE‑C™ silica hydride technology, providing fast equilibration, robust performance, and excellent reproducibility for LC‑MS method development.
Kit Composition
This development kit contains two analytical HPLC columns:
Shared specifications for both columns:
Internal Diameter: 2.1 mm
Length: 50 mm
Particle Size: 4 µm
Pore Size: 100 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The matched format ensures meaningful method‑development comparisons without introducing differences in column geometry or particle chemistry.
Application Focus: Metabolomics & LC‑MS Method Development
The two phases in this kit are complementary, addressing different regions of the metabolome:
Cogent Diamond Hydride™
HILIC‑style retention on a stable silica‑hydride surface
Strong retention for highly polar metabolites
Rapid equilibration and excellent LC‑MS compatibility
Cogent Bidentate C18™
Reversed‑phase retention with dual‑point C18 bonding
Enhanced stability relative to conventional bonded C18 phases
Effective for less polar and hydrophobic metabolites
Together, these columns provide broad metabolite coverage using a single, unified particle platform.
Why This Kit Is Ideal for Method Development
Using matched 2.1 × 50 mm columns in both chemistries delivers:
Direct HILIC vs. reversed‑phase comparison under identical geometry
Reduced solvent and sample consumption from the short 50 mm length
Robust, routine analytical operation with 4 µm particles
Consistent LC‑MS conditions, minimizing system‑to‑system bias
This kit is commonly selected for:
Untargeted and targeted metabolomics
Biomarker discovery and profiling
Small‑molecule screening
Exploratory LC‑MS method development
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
Compatible with standard analytical HPLC and LC‑MS systems capable of stable low‑flow delivery
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.