Metabolomics Development Kit, 4.6x100. 1 Cogent Diamond Hydride™ & 1 Cogent Bidentate C18™. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 43000‑10P Metabolomics Development Kit provides an economical and application‑ready pairing of two widely used TYPE‑C™ silica hydride HPLC columns for metabolomics and general small‑molecule LC workflows. This kit includes one Cogent Diamond Hydride™ HPLC Column and one Cogent Bidentate C18™ HPLC Column, allowing direct comparison of HILIC‑style and reversed‑phase separations using matched column geometry on standard analytical LC systems.
Both columns are manufactured with Cogent TYPE‑C™ silica hydride technology, offering fast equilibration, reproducible retention, and robust performance for routine method development and screening.
Kit Composition
This development kit contains two analytical HPLC columns:
Shared specifications for both columns:
Internal Diameter: 4.6 mm
Length: 100 mm
Particle Size: 4 µm
Pore Size: 100 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The matched column dimensions ensure meaningful method‑development comparisons without introducing differences in geometry, backpressure, or dwell‑volume effects.
Application Focus: Metabolomics & Analytical Method Development
The two stationary phases included in this kit provide complementary selectivity across polar and non‑polar compound classes:
Cogent Diamond Hydride™
HILIC‑style retention on a stable silica‑hydride surface
Strong retention of highly polar and ionic metabolites
Rapid equilibration and consistent method performance
Cogent Bidentate C18™
Reversed‑phase retention with dual‑point C18 bonding
Improved chemical stability compared to conventional bonded C18 phases
Effective for less polar and hydrophobic metabolites
Together, these columns enable broad metabolite coverage using a unified TYPE‑C™ surface chemistry, simplifying data comparison and method transfer.
Why Choose the 4.6 mm × 100 mm Format
The 4.6 mm ID × 100 mm length configuration is commonly selected for durable, high‑throughput analytical method development:
Improved resolution compared to 50 mm formats
Moderate run times suitable for screening and profiling
Standard analytical flow rates compatible with most LC systems
Well suited for UV, ELSD, CAD, and LC‑MS workflows
This format balances robustness, resolution, and ease of implementation on standard analytical platforms.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
Compatible with standard analytical HPLC and LC systems
No specialized low‑flow or UHPLC‑only hardware required
Flow conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.