Metabolomics Development Kit, 4.6x150. 1 Cogent Diamond Hydride™ & 1 Cogent Bidentate C18™. Cogent TYPE-C Brand. 1 PK.

Additional Info:

The 43000‑15P Metabolomics Development Kit provides an economical, application‑focused pairing of two widely used TYPE‑C™ silica hydride HPLC columns for metabolomics and broader small‑molecule workflows. This kit includes one Cogent Diamond Hydride™ HPLC Column and one Cogent Bidentate C18™ HPLC Column, allowing direct comparison of HILIC‑style and reversed‑phase separations using matched column geometry on standard analytical LC systems.

Both columns are manufactured with Cogent TYPE‑C™ silica hydride technology, delivering fast equilibration, reproducible retention, and robust performance across a wide range of analytical methods.

Kit Composition

This development kit contains two analytical HPLC columns:

Shared specifications for both columns:

  • Internal Diameter: 4.6 mm

  • Length: 150 mm

  • Particle Size: 4 µm

  • Pore Size: 100 Å

  • Stationary Phase Platform: Cogent TYPE‑C™ silica hydride

Using identical dimensions and particle architecture allows meaningful method‑development comparisons without introducing variability from column geometry or chromatography mode.

Application Focus: Metabolomics & LC Method Development

The two stationary phases in this kit provide complementary selectivity across polar and non‑polar chemical space:

Cogent Diamond Hydride™

  • HILIC‑style retention on a stable silica‑hydride surface

  • Strong retention for highly polar and ionic metabolites

  • Rapid equilibration and excellent method reproducibility

Cogent Bidentate C18™

  • Reversed‑phase retention with dual‑point C18 bonding

  • Enhanced stability versus conventional bonded C18 phases

  • Effective for less polar and hydrophobic metabolites

Together, these columns enable broad metabolite coverage using a consistent TYPE‑C™ surface chemistry.

Why Choose the 4.6 mm × 150 mm Format

The 4.6 mm ID × 150 mm length configuration is frequently selected for robust analytical method development on standard LC platforms:

  • Higher resolving power than 100 mm formats

  • Excellent peak capacity for complex samples

  • Standard analytical flow rates compatible with most LC systems

  • Well suited for UV, ELSD, CAD, and LC‑MS workflows

This format offers an effective balance between resolution, instrument compatibility, and operational simplicity.

System Requirements and Flow Rates

  • Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min

  • Compatible with standard analytical HPLC and LC systems

  • Requires no specialized low‑flow or UHPLC‑only hardware

Flow conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.

 

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