Metabolomics Development Kit, 2.1x150. 1 Cogent Diamond Hydride™ & 1 Cogent Bidentate C18™. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 43000‑15P‑2 Metabolomics Development Kit provides an economical and application‑focused pairing of two widely used TYPE‑C™ silica hydride HPLC columns for LC‑MS‑based metabolomics and small‑molecule analysis. This kit includes one Cogent Diamond Hydride™ HPLC Column and one Cogent Bidentate C18™ HPLC Column, enabling direct comparison of HILIC‑style and reversed‑phase separations using matched column geometry and particle technology.
Both columns are manufactured with Cogent TYPE‑C™ silica hydride, delivering fast equilibration, excellent reproducibility, and robust performance for metabolomics and related LC‑MS workflows.
Kit Composition
This development kit contains two analytical HPLC columns:
Shared specifications for both columns:
Internal Diameter: 2.1 mm
Length: 150 mm
Particle Size: 4 µm
Pore Size: 100 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The matched 2.1 × 150 mm format ensures meaningful method‑development comparisons without introducing variability from column geometry or particle chemistry.
Application Focus: Metabolomics & LC‑MS Method Development
The two stationary phases included in this kit address complementary regions of the metabolome:
Cogent Diamond Hydride™
HILIC‑style retention on a stable silica‑hydride surface
Strong retention of highly polar metabolites
Fast equilibration and excellent LC‑MS compatibility
Cogent Bidentate C18™
Reversed‑phase retention with dual‑point C18 bonding
Improved chemical stability compared to conventional bonded C18 phases
Effective for less polar and hydrophobic metabolites
Together, these columns provide broad metabolite coverage using a unified TYPE‑C™ surface chemistry, simplifying method development and data comparison.
Why Choose the 2.1 mm × 150 mm Format
The 2.1 mm ID × 150 mm length configuration is selected when higher resolving power and peak capacity are required compared to 50 mm and 100 mm columns:
Increased separation of closely eluting metabolites
Higher peak capacity for complex metabolomics samples
Reduced solvent consumption versus larger‑diameter columns
Well suited for routine and exploratory LC‑MS workflows requiring added resolution
The 4 µm particle size supports robust, repeatable operation on standard analytical and LC‑MS systems without the pressure demands of sub‑2 µm formats.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
Compatible with standard analytical HPLC and LC‑MS systems capable of stable low‑flow delivery
Flow conditions should be optimized based on mobile‑phase composition, temperature, and system pressure limits.