Metabolomics Development Kit, 2.o, 2x50. 1 Cogent Diamond Hydride 2.o™ & 1 Cogent Bidentate C18 2.o™ 2.1mm 50mm. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 43200‑05P‑2 Metabolomics Development Kit provides an economical and application‑focused way to evaluate two of the most commonly used TYPE‑C™ silica hydride stationary phases in LC‑MS‑based metabolomics workflows. This kit includes one Cogent Diamond Hydride™ 2.o HPLC Column and one Cogent Bidentate C18™ 2.o HPLC Column, allowing direct comparison of HILIC‑style and reversed‑phase separations using matched column geometry and particle technology.
Both columns are manufactured with Cogent TYPE‑C™ silica hydride, delivering robust performance, fast equilibration, and excellent reproducibility for metabolomics and related LC‑MS applications.
Kit Composition
This development kit contains two analytical HPLC columns:
Shared specifications for both columns:
Internal Diameter: 2.1 mm
Length: 50 mm
Particle Size: 2.2 µm
Pore Size: 120 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
This matched format allows meaningful method development comparisons without introducing differences in column dimensions or particle morphology.
Application Focus: Metabolomics & LC‑MS Method Development
The Diamond Hydride™ 2.o and Bidentate C18™ 2.o phases complement one another by addressing different regions of the metabolome:
Cogent Diamond Hydride™ 2.o
HILIC‑style retention with stable silica‑hydride surface
Strong retention for highly polar metabolites
Fast equilibration and excellent LC‑MS compatibility
Cogent Bidentate C18™ 2.o
Reversed‑phase retention with dual‑point C18 bonding
Enhanced stability compared to conventional bonded C18 phases
Effective for less polar and hydrophobic metabolites
Together, these two columns enable broad metabolite coverage and flexible method development using a single, unified particle platform.
Why This Kit Is Ideal for Method Development
Using a matched 2.o format for both columns provides several advantages:
Direct comparison between HILIC‑style and reversed‑phase methods
Reduced solvent and sample consumption due to short 50 mm length
High efficiency and near‑UHPLC performance from 2.2 µm particles
Consistent LC‑MS conditions, minimizing system‑to‑system bias
This kit is frequently selected for:
Untargeted and targeted metabolomics
Biomarker discovery
Small‑molecule profiling
Exploratory LC‑MS method development
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
Requires an HPLC or UHPLC system capable of accurate, stable low‑flow delivery
Ideal for LC‑MS and LC‑MS/MS systems designed for narrow‑bore columns
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.