Metabolomics Development Kit, 2.o, 2x100. 1 Cogent Diamond Hydride 2.o™ & 1 Cogent Bidentate C18 2.o™. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 43200‑10P‑2 Metabolomics Development Kit provides an application‑optimized pairing of two high‑efficiency TYPE‑C™ silica hydride HPLC columns for LC‑MS‑based metabolomics and small‑molecule profiling. This kit includes one Cogent Diamond Hydride™ 2.o HPLC Column and one Cogent Bidentate C18™ 2.o HPLC Column, allowing direct comparison of HILIC‑style and reversed‑phase separations using matched column geometry, particle size, and pore structure.
Both columns are manufactured using Cogent TYPE‑C™ silica hydride technology, delivering fast equilibration, excellent reproducibility, and robust performance under demanding LC‑MS conditions.
Kit Composition
This development kit contains two analytical HPLC columns:
Shared specifications for both columns:
Internal Diameter: 2.1 mm
Length: 100 mm
Particle Size: 2.2 µm
Pore Size: 120 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The matched 2.o format ensures meaningful method‑development comparisons without introducing variability from column dimensions or particle morphology.
Application Focus: Metabolomics & LC‑MS Method Development
The two phases included in this kit address complementary regions of the metabolome:
Cogent Diamond Hydride™ 2.o
HILIC‑style retention on a stable silica‑hydride surface
Strong retention for highly polar metabolites
Fast equilibration and excellent LC‑MS compatibility
Cogent Bidentate C18™ 2.o
Reversed‑phase retention with dual‑point C18 bonding
Enhanced stability compared to conventional bonded C18 phases
Effective for less polar and hydrophobic metabolites
Together, these columns provide broad metabolite coverage using a single, unified TYPE‑C™ particle platform.
Why Choose the 2.1 mm × 100 mm 2.o Format
The 2.1 mm ID × 100 mm length with 2.2 µm particles is frequently selected when higher resolution than 50 mm columns is required while maintaining solvent efficiency and LC‑MS compatibility:
Higher resolving power and peak capacity than 50 mm formats
Reduced solvent consumption relative to larger‑diameter columns
Near‑UHPLC performance using standard LC‑MS‑capable systems
Ideal for complex metabolomic samples requiring confident separation
This format is well suited for both exploratory and confirmatory LC‑MS workflows.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
Requires an LC or UHPLC system capable of accurate and stable low‑flow delivery
Optimized for LC‑MS and LC‑MS/MS platforms
Flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.