Metabolomics Development Kit, 2.o, 2x150. 1 Cogent Diamond Hydride 2.o™ & 1 Cogent Bidentate C18 2.o™. Cogent TYPE-C Brand. 1 PK.
Additional Info:
The 43200‑15P‑2 Metabolomics Development Kit provides an application‑optimized pairing of two high‑efficiency TYPE‑C™ silica hydride HPLC columns designed for high‑resolution LC‑MS‑based metabolomics and small‑molecule profiling. This kit includes one Cogent Diamond Hydride™ 2.o HPLC Column and one Cogent Bidentate C18™ 2.o HPLC Column, enabling direct comparison of HILIC‑style and reversed‑phase separations using matched column dimensions, particle size, and pore structure.
Both columns are manufactured with Cogent TYPE‑C™ silica hydride technology, delivering stable retention, rapid equilibration, and excellent reproducibility for demanding LC‑MS metabolomics workflows.
Kit Composition
This development kit contains two analytical HPLC columns:
Shared specifications for both columns:
Internal Diameter: 2.1 mm
Length: 150 mm
Particle Size: 2.2 µm
Pore Size: 120 Å
Stationary Phase Platform: Cogent TYPE‑C™ silica hydride
The matched 2.o format ensures meaningful method‑development comparisons without introducing variability from column geometry, particle morphology, or surface chemistry.
Application Focus: Metabolomics & LC‑MS Method Development
The two phases included in this kit address complementary regions of the metabolome and are frequently used together in metabolomics workflows:
Cogent Diamond Hydride™ 2.o
HILIC‑style retention on a stable silica‑hydride surface
Strong retention for highly polar metabolites
Fast equilibration and excellent LC‑MS compatibility
Cogent Bidentate C18™ 2.o
Reversed‑phase retention with dual‑point C18 bonding
Enhanced chemical stability relative to conventional bonded C18 phases
Effective for less polar and hydrophobic metabolites
Together, these columns provide broad metabolite coverage using a unified TYPE‑C™ particle platform, simplifying method development and data comparison.
Why Choose the 2.1 mm × 150 mm 2.o Format
The 2.1 mm ID × 150 mm length combined with 2.2 µm particles is selected when maximum analytical resolution and peak capacity are required:
Higher resolving power than 50 mm and 100 mm formats
Increased peak capacity for complex metabolomic samples
Reduced solvent consumption compared to larger‑diameter columns
Near‑UHPLC performance while maintaining LC‑MS compatibility
This format is ideal when separation confidence and metabolite coverage are prioritized over shortest run time.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
Requires an LC or UHPLC system capable of accurate, stable low‑flow delivery
Optimized for LC‑MS and LC‑MS/MS platforms handling narrow‑bore columns
Flow conditions should be optimized based on mobile‑phase viscosity, temperature, and system pressure limits.