Sarcosine Analysis by LC-MS Separation from Isobaric B-Alanine Using Cogent Diamond Hydride - AppNote
May 1, 2012
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Date: 1-MAY-2012 Last Updated: 19-SEPTEMBER-2026
Separation of Potential Urine Biomarker from Isobaric ß-AlanineThis developed LCMS method can separate Sarcosine from Beta- Alanine in serum and urine samples without using labor intensive sample derivatization. Since Sarcosine is considered a potential biomarker for prostate cancer risk and aggressiveness, it is essential to resolve and accurately quantify this compound in the presence of isobaric (same m/z ) Beta-Alanine.
The developed method is Sensitive, Specific, Quantitative, and Reproducible (%RSD = 0.1). It can be used in large scale studies with numerous samples (high throughput of the method due to simple sample preparation).
Peaks: 1. Sarcosine, 2. ß-Alanine
Method ConditionsColumn: Cogent Diamond Hydride™, 4 μm, 100 Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150 mm
Mobile Phase:
- A: 50% Isopropyl Alcohol / 50% DI Water / 0.1% Acetic Acid
- B: 97% Acetonitrile / 3% DI Water / 0.1% Acetic Acid
| Time (minutes) | %B |
|---|---|
| 0 | 75 |
| 3 | 75 |
| 4 | 65 |
| 5 | 65 |
| 10 | 20 |
| 12 | 75 |
Temperature: 50˚ C
Post Time: 5 minutes
Flow rate: 0.6 mL/minute
Detection: ESI – POS - Agilent 6210 MSD TOF Mass Spectrometer
Injection vol.: 1 μL
Sample Preparation: 10 mg/L each of Sarcosine and Beta-Alanine in 50:50 A:B
Note: When Reversed Phase Columns were evaluated for their ability to separate Sarcosine from Beta-Alanine, both compounds eluted at the solvent front and were not separated. To achieve separation, a very intensive sample preparation has to be employed (e.g. derivatization) when using RP methods.
