Vancomycin Analyzed with HPLC - AppNote
May 18, 2012
/
/
/
Retention of a Highly Polar Antibiotic
As a highly polar compound, Vancomycin is difficult to retain with Reversed Phase Methods. In this Method the compound retains very well as illustrated in the Figure. In addition, the repeatability of the analysis, which is demonstrated by the overlay of five consecutive runs, is excellent. The Mobile Phase used is LCMS compatible and the low equilibration time after the Gradient allows for a rapid and Robust Analysis.
Column: Cogent Diamond Hydride™, 4 µm, 100 Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase:
Post Time: 2 minutes
Injection vol.: 5 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 210 nm
Sample Preparation:
Note: Vancomycin is a glycosylated nonribosomal peptide antibiotic used to treat Colitis. Vancomycin is often used as a drug of last resort when other antibiotics are rendered ineffective due to developed resistance of bacteria. It is a natural product isolated from Amycolatopsis Orientalis.
Attachment No 159 Vancomycin Analyzed with HPLC pdf 0.6 Mb Download File
As a highly polar compound, Vancomycin is difficult to retain with Reversed Phase Methods. In this Method the compound retains very well as illustrated in the Figure. In addition, the repeatability of the analysis, which is demonstrated by the overlay of five consecutive runs, is excellent. The Mobile Phase used is LCMS compatible and the low equilibration time after the Gradient allows for a rapid and Robust Analysis.
Peak: Vancomycin
Method ConditionsColumn: Cogent Diamond Hydride™, 4 µm, 100 Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase:
- A: DI Water / 0.1% Formic Acid
- B: Acetonitrile / 0.1% Formic Acid
| Time (minutes) | %B |
|---|---|
| 0 | 70 |
| 6 | 10 |
| 7 | 70 |
Post Time: 2 minutes
Injection vol.: 5 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 210 nm
Sample Preparation:
- Stock Solution: 1 mg / mL Vancomycin HCl in 50:50 Solvent A / Solvent B diluent. The solution was filtered through a 0.45 µm Nylon Syringe Filter (MICROSOLV Tech Corp).
- Working Solution: Stock solution was diluted 1:100 with 50:50 Solvent A / Solvent B mixture.
Note: Vancomycin is a glycosylated nonribosomal peptide antibiotic used to treat Colitis. Vancomycin is often used as a drug of last resort when other antibiotics are rendered ineffective due to developed resistance of bacteria. It is a natural product isolated from Amycolatopsis Orientalis.
Attachment No 159 Vancomycin Analyzed with HPLC pdf 0.6 Mb Download File