Glyburide and Metformin Analyzed with HPLC - AppNote
May 23, 2012
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Separation of Highly Polar and Non-Polar Compounds in one Isocratic run
In this Method, the polar compound, Metformin, and the nonpolar compound Glyburide, can be retained on the same Stationary Phase (see A & B). Depending on the Mobile Phase composition either Metformin or Glyburide can be retained longer.
Method Conditions
Column: Cogent Bidentate C18™, 4 µm, 100 Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase:
-- A: 15% DI Water / 85% Acetonitrile / 0.5% Formic Acid
-- B: 50% DI Water / 50% Acetonitrile / 0.5% Formic Acid
Injection vol.: 1 µL
Flow rate: 0.5 mL / minute
Detection: UV @ 254 nm
Sample Preparation: Stock Solution: 100µg / µL Glyburide and Metformin
Notes: Elution order was confirmed by LCMS, APCI+, with single ion monitoring Metformin (m/z 130) and Glyburide (m/z 369).
Attachment No 08 Glyburide and Metformin Analyzed with HPLC pdf 0.2 Mb Download File
In this Method, the polar compound, Metformin, and the nonpolar compound Glyburide, can be retained on the same Stationary Phase (see A & B). Depending on the Mobile Phase composition either Metformin or Glyburide can be retained longer.
Method Conditions
Column: Cogent Bidentate C18™, 4 µm, 100 Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase:
-- A: 15% DI Water / 85% Acetonitrile / 0.5% Formic Acid
-- B: 50% DI Water / 50% Acetonitrile / 0.5% Formic Acid
Injection vol.: 1 µL
Flow rate: 0.5 mL / minute
Detection: UV @ 254 nm
Sample Preparation: Stock Solution: 100µg / µL Glyburide and Metformin
Notes: Elution order was confirmed by LCMS, APCI+, with single ion monitoring Metformin (m/z 130) and Glyburide (m/z 369).
Attachment No 08 Glyburide and Metformin Analyzed with HPLC pdf 0.2 Mb Download File