Acrylamide Analyzed with HILIC HPLC - AppNote
August 13, 2012
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Date: 13-AUGUSUT-2012 Last Updated: 30-AUGUST-2026
Easy & Precise Retention of a Very Polar CompoundAcrylamide can be difficult to retain with conventional Reversed Phase Methods due to its polar nature. With this Method however, Retention is readily achievable using a simple, Isocratic Mobile Phase. The overlay of two Chromatograms using two different lots of HPLC Columns in the Figure below illustrates the Reproducibility and Robustness of this Method.
Peak: Acrylamide
Method ConditionsColumn: Cogent Diamond Hydride™, 4 μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase: Acetonitrile with 0.1% Formic Acid
Injection vol.: 1 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 205 nm
Sample Preparation: 0.1 mg / mL Acrylamide in Mobile Phase as the Diluent.
t 0 : 1.0 minute
Note: Acrylamide is a monomer used to synthesize polyacrylamides. It was reported to be present in certain food products in 2002. This has been cause for concern as the monomer form is a known carcinogen and neurotoxin. As such, quantitation of this analyte is of importance in a variety of fields.
