Cyclobenzaprine Retention with an Inverse Gradient - AppNote
March 26, 2013
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Date: 26-MARCH-2013 Last Updated: 20-AUGUST-2026
HILIC Retention of a Hydrophobic Compound
This Application Note shows an example of a hydrophobic analyte retention in HILIC Like Gradient HPLC. Although Cyclobenzaprine has a logP of approximately 4.6–4.9, it is well retained with a Symmetrical Peak Shape.Data from two production lots of Columns is shown to illustrate the Reproducibility of the Method.
Peak:
Cyclobenzaprine
Column: Cogent Diamond Hydride™, 4 μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase:
-- A: DI Water with 0.1% Formic Acid (v/v)
-- B: Acetonitrile with 0.1% Formic Acid (v/v)
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 95 |
| 1 | 95 |
| 6 | 40 |
| 7 | 95 |
Flow rate: 1.0 mL / minute
Detection: UV @ 224 nm
Sample Preparation: 10 mg strength Cyclobenzaprine Tablet was ground and added to a 25 mL volumetric flask. A diluent of 50:50 Solvent A / Solvent B was added and the flask was sonicated for 30 minutes. It was then diluted to mark, mixed, and filtered with a 0.45 μm Nylon Syringe Filter (MICROSOLV Tech Corp.). A portion of the filtrate was diluted 1:10 for HPLC Injections.
t 0 : 0.9 minutes
Note: Cyclobenzaprine is a muscle relaxant and used to treat Fibromyalgia. Cyclobenzaprine is structurally related to first-generation tricyclic antidepressants. It is marketed under the trade name Flexeril®.
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