Date: 10-JULY-2013   Last Updated: 3-NOVEMBER-2025

Improve Peak Shapes in LC-MS: Add 10 µM EDTA to the Mobile Phase

Poor peak shapes—especially for polyprotic acids and nucleotides—can often be traced back to metal contamination. These metals may leach from:

• Glass solvent bottles
• Autosampler vials
• Internal components of the LC-MS system

The extent of this issue can vary significantly between instruments, so it's important to identify the specific source of peak distortion in your setup.

Suggested Solution

To mitigate metal-related peak tailing or distortion, consider adding 10 µM EDTA to your mobile phase when using Cogent TYPE-C HPLC columns. These chelating agents can bind trace metals, improving peak symmetry and reproducibility.

Note: Always verify compatibility with your method,column and instrument before modifying mobile phase composition.