HILIC Phase Support - Information
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  • Technical guidance on HILIC chromatography and HILIC column applications for polar compound analysis. Resources cover mobile phase chemistry, retention behavior, sample preparation considerations, LC-MS optimization, method development, and troubleshooting techniques for improving selectivity, sensitivity, and reproducibility.


Acetone as a Mobile Phase Solvent in HILIC Methods - Tech Information
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Acid Concentration Optimization in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Ammonium Acetate and Ammonium Formate in Acetonitrile Rich HILIC Mobile Phases - Tech Information
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Ammonium Acetate in LC-MS Methods Preventing Source Contamination and Signal Loss - Tech Information
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Ammonium Acetate or Ammonium Formate in Diamond Hydride Methods - Tips and Suggestions
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Ammonium Acetate or Triethylamine TEA Use with Cogent HPLC Columns - Tips and Suggestions
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Ammonium Acetate Use in HILIC LC-MS Methods with TYPE-C Columns - Tech Information
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Ammonium Fluoride in HILIC HPLC and LC-MS Methods Using TYPE-C Columns - Tech Information
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Ballistic Gradients in HILIC Methods Using TYPE-C Columns - Tech Information
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Baseline Stability in HILIC Methods Using Cogent Diamond Hydride Columns - Tech Information
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Broad or Split Peaks in HILIC When Using Methanol ‑ Rich Diluents - Tips and Suggestions
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Buffer Concentration Effects on Acid Retention in HILIC Methods Using TYPE-C™ Columns - Tech Information
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Do You Really Need High pH for Retention in HPLC - Tech Information
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Effects of Mobile Phase Ionic Strength in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Eliminating Retention Time Drift in LC-MS HILIC Methods Using Diamond Hydride Columns - Tech Information
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Flat Baselines in Gradient HPLC Without Blank Subtraction - Tech Information
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Glutamine Glutamic Acid and Aspartic Acid Separation - Tech Information
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High Injection Volume Effect on Column Performance in HILIC Methods - Tech Information
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HILIC and Reversed Phase Retention Simultaneously on TYPE-C Columns - Tech Information
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HILIC Column Robustness and the Advantages of TYPE-C Technology - Tech Information
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HILIC Efficiency and the Impact of TYPE-C Silica Hydride Technology - Tech Information
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HILIC Methods Using Cogent TYPE-C Columns Compared to Conventional HILIC Columns - Tech Information
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HILIC Retention Mechanism on TYPE-C Silica Hydride Columns Elucidated - Tech Information
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HILIC Retention of Polar Compounds Using TYPE-C™ Silica Columns - Tech Information
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HILIC Reversed Phase and Normal Phase Chromatography Defined - HPLC Primer
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HILIC Separations of Polar Compounds Using TYPE-C Columns - HPLC Primer
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HILIC Separations of Polar Compounds Using TYPE-C Columns - Tech Information
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HPLC Columns You May Not Know About That Can Be Very Helpful - White Paper
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Hydrophobicity and Column Selection Guide for Cogent TYPE-C HPLC Columns - Tech Information
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Improving Accuracy and Precision for Polar Compound Analysis Using HILIC Methods and TYPE-C Columns - Tech Information
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Improving LC-MS Sensitivity with HILIC Methods for Polar Compound Analysis - Tech Information
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Improving Metformin Reproducibility and Peak Shape in Plasma Sample Analysis - Tech Information
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Improving Peak Shape and Retention for Organic Acids in HILIC Methods Using Cogent Diamond Hydride Columns - Tech Information
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Improving Peak Shape for Phosphorylated Compounds in HILIC Methods Using TYPE-C Columns - Tech Information
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Improving Peak Shape in HILIC Methods - Tech Information
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Improving Polar Compound Analysis with TYPE-C HILIC Columns for LC and LC-MS - Tech Information
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Injection Techniques for Aqueous Samples in HILIC Methods - Tech Information
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Ion‑Pairing Reagents with Cogent Diamond Hydride Columns - Tech Information
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Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
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LC-MS Challenges Associated with High-Salt HILIC Mobile Phases - Tech Information
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Maleic Hydrazide Method Development - Tips & Suggestions
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Maximum Concentration of Ammonium Fluoride in a Mobile Phase - Tips and Suggestions
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Maximum Salt Concentration Recommendations for Cogent TYPE-C HPLC Columns - Tech Information
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Metabolomics Research Applications Using Cogent TYPE-C Columns
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Methanol in Place of Acetonitrile in HPLC Methods - Tech Information
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Mobile Phase Additives and Cogent Diamond Hydride HPLC Columns - Tech Information
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Phosphorylated Compounds Analyzed with Cogent Diamond Hydride Columns - Tech Information
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Platinum Catalyst Residue Evaluation on Cogent TYPE C Silica Stationary Phases - Tech Information
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Polar and Non-Polar Peptide Analysis by LC-MS Using HILIC Methods - AppNote
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Polar Compound Method Development Using Cogent TYPE-C HILIC Columns - Tech Information
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Purge Metals from HPLC System Using EDTA - How To
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Retention Behavior of Triphenylamine in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Retention of Ionized Polar Compounds in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Selecting Organic Solvents for HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Selecting the Optimal Gradient Direction for Mixed-Polarity Compounds Using Cogent TYPE-C Columns - Tech Information
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Separating Hydrophilic and Hydrophobic Compounds in the Same HILIC Method - Tech Information
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Separating Polar and Non-Polar Compounds with Cogent TYPE-C Silica Columns
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Serum Sample Analysis Using Cogent Diamond Hydride Columns for LC-MS - Tech Information
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Sodium Ions and Sodium Succinate Analysis in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Solvent Strength and Mobile Phase Selection for HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Steroid Analysis Using HILIC and Reversed Phase HPLC Columns - Tech Information
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Storage Conditioning and Solvent Transition Guidelines for Cogent TYPE-C HPLC Columns - Tech Information
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Switching Between Reversed Phase and Normal Phase Methods on Cogent TYPE C Silica Columns - Tech Information
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Tandem HPLC Columns in One Separation for Polar and Non Polar Compounds - Tips and Suggestions
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Understanding Normal Phase Reversed Phase and HILIC Retention Modes with TYPE-C Columns - Tech Information
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Urea Retention Challenges with the Cogent Bidentate C18 Column - AppNote
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Using a Dedicated Reversed Phase HPLC System for HILIC Methods Potential Problems and How to Avoid Them - Tech Information
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Using Deactivator Agents with Cogent TYPE‑C HPLC Columns - Tech Information
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Using EDTA in Mobile Phases to Improve Peak Shape for Metal-Sensitive Compounds - Tech Information
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Using Isopropanol IPA in HILIC Mobile Phases with Cogent TYPE-C Columns - Tech Information
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Using Triethylamine TEA in HILIC Methods for Simple Sugar Analysis - Tech Information
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When and Why to Use PTFE Teflon Mobile Phase Reservoir Bottles for HPLC and LC-MS - Tech Information
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Why Polar Compounds Often Show Poor Retention on Traditional Reversed-Phase Columns - HPLC Primer
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Why Tanaka Plots Are Not Suitable for Evaluating HILIC Column Performance - Tech Information
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