Benzodiazepine Drug Screening in Urine by SPE and LC-MS Using a Diamond Hydride Column - AppNote
May 1, 2014
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Date: 1-MAY-2014 Last Updated: 30-AUGUST-2026
1,4-Benzodiazepine compounds from Urine Samples were analyzed successfully after Solid Phase Extraction (SPE).Four available compounds (shown below) were well retained and separated. The procedure could be used for determination of this class of compounds in urine samples and other body fluids.
Peaks:
1. Temazepam
301.0739 m/z [M+H]+
2. Diazepam
285.0790 [M+H]+
3. Nordiazepam
271.0633 [M+H]+
4. Midazolam
326.0855 [M+H]+
Column: Cogent Diamond Hydride 2.0™, 2.2 µm, 120Å
Catalog No.: 70200-05P-2
Dimensions: 2.1 x 50 mm
Solvents:
---- A: DI H20 / 0 .1% formic Acid (v/v)
---- B: Acetonitrile/ 0.1% formic Acid (v/v)
Gradient:
| time | %B |
| 0 | 85 |
| 6 | 70 |
| 7 | 20 |
| 9 | 20 |
| 10 | 85 |
Flow rate: 0.4 ml / minute
Detection: ESI – POS – Perkin Elmer AxION 2 TOF Mass Spectrometer
Samples:
---- Extraction method: Spiked urine sample was loaded into SPE cartridge I (Clean Screen Xcel™ purchased from UCT Bristol, PA, USA) and eluted with 0.78mL of acetonitrile, 200µL of 2-propanol, 20µL of ammonia. After the elution, the sample was dried under N2 gas and dissolved in 100µL of 50:50 methanol / DI H2O / 0.1% formic acid.
NOTE: Before injection, the 10 ppm spiked sample was filtered through a 0 .45µm AQ™ Brand Nylon Syringe Filter
