HPLC Method Transferred to Near UHPLC - AppNote
June 10, 2014
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Date: 10-JUNE-2014 Last Updated: 7-FEBRUARY-2026
Use of near UHPLC column for improved results
This challenging compound has several amine groups and can be problematic to analyze by HPLC. Here a good peak shape is obtained using the Cogent Diamond Hydride 2.o Column. The efficiency is higher on the 2.2µm phase compared to a standard 4µm Column, leading to increased sensitivity. The method conditions are compatible with LCMS as well.
Columns:
Post time: 3 minutes
Injection vol.: 0.2 µL
Flow rate: 0.3 mL/minutes
Detection: UV @ 230 nm
Sample: 4 mg strength Tizanidine HCl tablet was ground and weighed in a 10 mL Volumetric Flask. A portion of 50/50 Solvent A / Solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45 µm Nylon Syringe Filter (MICROSOLV Tech Corp.).
t 0 : 0.6 minutes
Note: Tizanidine is a centrally acting A2-Adren- Ergic agonist used to treat spasms, cramping, tightness of muscles, and related conditions. It is available under the trade name Zanaflex® as well as generic versions.
Attachment Tizanidine HCI Method Transfer pdf Download File
Use of near UHPLC column for improved results
This challenging compound has several amine groups and can be problematic to analyze by HPLC. Here a good peak shape is obtained using the Cogent Diamond Hydride 2.o Column. The efficiency is higher on the 2.2µm phase compared to a standard 4µm Column, leading to increased sensitivity. The method conditions are compatible with LCMS as well.
Peak: Tizanidine HCI
Method ConditionsColumns:
- Fig. A: Cogent Diamond Hydride 2.o™, 120Å
- Fig. B: Cogent Diamond Hydride™, 4µm, 100Å
- Fig. A: 70200-05P-2;
- Fig. B: 70000-05P-2
- Fig. A: 2.1 x 50 mm
- Fig. B: 2.1 x 50 mm
- A: DI Water / 0.1% Formic Acid (v/v)
- B: Acetonitrile / 0.1% Formic Acid (v/v)
| Time (minutes) | %B |
|---|---|
| 0 | 95 |
| 1 | 95 |
| 6 | 40 |
| 7 | 95 |
Post time: 3 minutes
Injection vol.: 0.2 µL
Flow rate: 0.3 mL/minutes
Detection: UV @ 230 nm
Sample: 4 mg strength Tizanidine HCl tablet was ground and weighed in a 10 mL Volumetric Flask. A portion of 50/50 Solvent A / Solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45 µm Nylon Syringe Filter (MICROSOLV Tech Corp.).
t 0 : 0.6 minutes
Note: Tizanidine is a centrally acting A2-Adren- Ergic agonist used to treat spasms, cramping, tightness of muscles, and related conditions. It is available under the trade name Zanaflex® as well as generic versions.
Attachment Tizanidine HCI Method Transfer pdf Download File