Tizanidine HCl Tablet Precision by HPLC - AppNote
September 1, 2016
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Excellent Run to Run Precision
Tizanidine has several amine groups which can present problems to chromatographers. Residual silanols on the surface of ordinary Silica-based Columns can electrostatically interact with these groups, causing peak tailing.
The Cogent Amide Column, used in the Application Note is virtually free of residual silanols and therefore a symmetrical peak shape can be obtained. An overlay of five consecutive runs demonstrates the run- to-run precision when using these columns.
Column: Cogent Amide™, 4μm, 100Å
Catalog No.: 40036-05P
Dimensions: 4.6 x 50mm
Mobile Phase:
-- A: 90% DI Water / 10% Acetonitrile / 0.1% Formic Acid (v/v)
-- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
Flow rate: 1.0 mL/minute
Detection: UV @ 230nm
Injection vol.: 3μL
Sample Preparation:
4 mg strength Tizanidine HCl tablet was ground and weighed in a 10 mL volumetric flask. A portion of 50/50 solvent A/ solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45µm Nylon syringe filter (MICROSOLV Tech Corp). An aliquot was then diluted 1:10 with a diluent of 10% solvent A / 90%.
Note: Tizanidine is a centrally acting α2- adrenergic agonist used to treat spasms, cramping, tightness of muscles, and related conditions. It is available under the trade name Zanaflex® as well as generic versions.
Attachment No 356 Tizanidine Amide.pdf 0.5 Mb Download File
Tizanidine has several amine groups which can present problems to chromatographers. Residual silanols on the surface of ordinary Silica-based Columns can electrostatically interact with these groups, causing peak tailing.
The Cogent Amide Column, used in the Application Note is virtually free of residual silanols and therefore a symmetrical peak shape can be obtained. An overlay of five consecutive runs demonstrates the run- to-run precision when using these columns.
Peak:
Tizanidine
Column: Cogent Amide™, 4μm, 100Å
Catalog No.: 40036-05P
Dimensions: 4.6 x 50mm
Mobile Phase:
-- A: 90% DI Water / 10% Acetonitrile / 0.1% Formic Acid (v/v)
-- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
| Time (Minutes) | %B |
|---|---|
| 0 | 90 |
| 2 | 90 |
| 6 | 40 |
| 7 | 90 |
Detection: UV @ 230nm
Injection vol.: 3μL
Sample Preparation:
4 mg strength Tizanidine HCl tablet was ground and weighed in a 10 mL volumetric flask. A portion of 50/50 solvent A/ solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45µm Nylon syringe filter (MICROSOLV Tech Corp). An aliquot was then diluted 1:10 with a diluent of 10% solvent A / 90%.
Note: Tizanidine is a centrally acting α2- adrenergic agonist used to treat spasms, cramping, tightness of muscles, and related conditions. It is available under the trade name Zanaflex® as well as generic versions.
Attachment No 356 Tizanidine Amide.pdf 0.5 Mb Download File