Date: 24-July-2025   Last Updated: 1 -August-2025   

Assembly Procedure for Cogent Guard Column Holder Installation

1. Component Inspection

• Verify the guard cartridge matches specified requirements and is compatible with the HPLC Column stationary phase.

2. Holder Preparation

• Disassemble the guard column holder if necessary.

3. Cartridge Insertion

• Place the guard cartridge into the holder cavity. Note: Flow direction is not defined for cartridges in this configuration.

  • Do not apply excessive force during insertion.

4. Cartridge Securing

• Reassemble the holder with thread alignment.
• ​ Finger-tighten securely, then apply an additional 1/8" clockwise turn using a 3/8" wrench  to ensure seal integrity.

5. Leak Testing

• Connect the holder to the system using standard tubing.
• ​ Conduct a low-pressure test to evaluate seal performance.

Leak Detection Method: Use the folded edge of a Kimwipe (lint-free paper) to touch the junction area of the column holder. If solvent is absorbed, incrementally tighten fittings in small amounts and retest until leakage ceases.

6. Setup Finalization

• Record cartridge details in the maintenance log (e.g., part number, installation date).
• Perform the r ecommended column performance test to establish baseline values for pressure and chromatographic behavior. This enables future verification if performance changes are observed.