Date: 10-FEB-2022   Last Updated: 24-AUGUST-2026

This traightforward method provides effective retention and good resolution for L‑ascorbic acid and erythorbic acid , two stereoisomeric acidic compounds with identical molecular formulas and closely matching physicochemical properties. Notably, strong separation is achieved even though both analytes exhibit very low hydrophobicity (log P ≈ −1.6),

 

Peaks:
1. D- Isoascorbic acid,  2. L- Ascorbic acid

Method Conditions
Column:  Cogent Diamond Hydride™, 4 µm, 100 Å
Catalog No.:  70000-15P
Dimensions: 4.6 x 150 mm 

Mobile Phase: 100% Acetonitrile + 0.1% Formic Acid
Flow Rate: 1.0mL / minute
Injection Volume: 1uL

Detection: UV 254 nm
Injection vol.: 1 μL

Sample Preparation: D- Isoascorbic Acid and L- Ascorbic Acid in 1.0 mg / mL in diluent of 50% Acetonitrile / 50% DI Water (v/v)
t0: 1.8 minutes

Note:  Vitamin C exists in different stereochemical forms. The one your body actually uses is L‑ascorbic acid, the naturally occurring form found in fruits and vegetables that helps prevent scurvy.