Gemcitabine Assay Analyzed with HPLC – AppNote
August 17, 2021
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A
Reliable
Method for a
Chemotherapeutic
Drug
This Gemcitabine Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.4%. This Method shows consistency and reliability for analysis of this Nucleoside Chemotherapeutic.
Note: Gemcitabine is a nucleoside analog and a chemotherapeutic agent. It is used as adjunct therapy in the treatment of certain types of ovarian cancer, non-small cell lung carcinoma, metastatic breast cancer, and as a single agent for pancreatic cancer.
This Gemcitabine Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.4%. This Method shows consistency and reliability for analysis of this Nucleoside Chemotherapeutic.
Peak:
Gemcitabine
Met
hod Conditions:
Column: Cogent RP C8™, 5 um, 150 Å
Catalog No.: 68508-25P
Dimensions: 4.6 x 250 mm
Mobile Phase: 13.8 g of Monobasic Sodium Phosphate and 2.5 ml of Phosphoric Acid in 1L of Water.
Injection vol.:
20 μL
Flow rate:
1.2 mL / minute
Detection:
UV @ 275nm
Note: Gemcitabine is a nucleoside analog and a chemotherapeutic agent. It is used as adjunct therapy in the treatment of certain types of ovarian cancer, non-small cell lung carcinoma, metastatic breast cancer, and as a single agent for pancreatic cancer.
his Method was developed by and is presented courtesy of ARL- Eutech Scientific Services, Inc.