Sulfur Analyzed with HPLC - AppNote
April 25, 2023
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Analyzing Elemental
Sulfur Content
A simple and reproducible method has been developed for analysis of elemental Sulfur . The data below, (an overlay of 5 chromatograms) illustrates how the compound can be adequately retained in HPLC with good precision and peak shapes using this straightforward method.
Column: Cogent Bidentate C18™, 4 μm, 100 Å.
Catalog No.: 40018-15P
Dimensions: 4.6 x 150 mm
Mobile Phase: (90:10) Acetonitrile / DI water with 0.1% formic acid.
Injection vol.: 3 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 263 nm
Sample Preparation: 0.5 mg / mL Sulfur (7704-34-9) in DCM
%RSD: <0.1%
t0: 1.7 minutes
K’: 5.2
Notes: This method could be used for determination of elemental Sulphur in soils, using DCM as the extracting solvent.
Note 2: Capacity was determined using the following equation: k = (tR-t0) / tO
tR = Retention time of an analyte peak
tO = Retention time of non-retained peak
A simple and reproducible method has been developed for analysis of elemental Sulfur . The data below, (an overlay of 5 chromatograms) illustrates how the compound can be adequately retained in HPLC with good precision and peak shapes using this straightforward method.
Peak: Sulfur
Method Conditions:Column: Cogent Bidentate C18™, 4 μm, 100 Å.
Catalog No.: 40018-15P
Dimensions: 4.6 x 150 mm
Mobile Phase: (90:10) Acetonitrile / DI water with 0.1% formic acid.
Injection vol.: 3 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 263 nm
Sample Preparation: 0.5 mg / mL Sulfur (7704-34-9) in DCM
%RSD: <0.1%
t0: 1.7 minutes
K’: 5.2
Notes: This method could be used for determination of elemental Sulphur in soils, using DCM as the extracting solvent.
Note 2: Capacity was determined using the following equation: k = (tR-t0) / tO
tR = Retention time of an analyte peak
tO = Retention time of non-retained peak