4-Methylamino-butyric Acid Analyzed by HPLC- AppNote
April 23, 2021
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Date: 23-APRIL-2021 Last Updated: 31-AUGUST-2026
A gamma-Aminobutyric Acid (GABA) Derivative
This challenging polar compound is easily retained in this simple Method where repeatability of the data is excellent with %RSD<0.1 for the Retention Times.
Peak: 4-(Methylamino)butyric Acid
Met hod Conditions:Column: Cogent Diamond Hydride™, 4 μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 4.6 x 150 mm
Mobile Phase:
-- A: DI Water with 0.1% Formic Acid
-- B: Acetonitrile with 0.1% Formic Acid
| Time (minutes) | %B |
|---|---|
| 0 | 80 |
| 5 | 30 |
| 6 | 30 |
| 7 | 80 |
Injection vol.: 1 μL
Flow rate: 1.0 mL / minute
Detection: ELSD (Evaporative Light Scattering Detector)
Sample Preparation: 4-(Methylamino) Butyric Acid 0.1 mg / mL in 50:50 Acetonitrile / DI Water
Note: 4-(Methyamino)butyric acid is a GABA (gamma Aminobutyric Acid) derivative and product of N-methyl-2-pyrrolidione. It inhibits L-Carnitine from undergoing beta-oxidation in mammals. In bacteria it is a product of nicotine catabolism. It is found in skin products to prevent wrinkles.
