HPLC Column, Diamond Hydride, 4 um, 2.1 mm ID x 150 mm Length, 100 A. Cogent TYPE-C Brand. 1 EA.

Additional Info:

The 70000‑15P‑2 Diamond Hydride HPLC Column is designed for applications that demand maximum chromatographic resolution in HILIC separations, while maintaining compatibility with LC‑MS and LC‑MS/MS systems. The combination of a narrow internal diameter and extended column length provides enhanced separation efficiency for complex polar analyte mixtures.

When to Choose the 150 mm Length

The 150 mm column length significantly increases the number of theoretical plates compared to 50 mm and 100 mm formats, enabling:

  • Higher peak capacity for complex sample matrices

  • Improved resolution of closely eluting polar compounds

  • Greater confidence in analyte identification and quantitation

This makes the column particularly well suited for metabolomics, biomolecule analysis, and demanding research or regulated workflows where separation quality is prioritized over run time.

Advantages of the 2.1 mm ID Format

The 2.1 mm internal diameter supports:

  • Lower operating flow rates, ideal for electrospray‑based MS detection

  • Reduced solvent consumption compared to standard‑bore columns

  • Improved ionization efficiency and cleaner MS sources

For these reasons, this configuration is often chosen for LC‑MS methods when increased resolution is required, without migrating to UHPLC‑specific particle sizes.

Diamond Hydride™ Stationary Phase Performance

Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase offers:

  • Excellent selectivity for polar and moderately polar analytes

  • Faster equilibration than conventional bonded silica HILIC phases

  • Robust column lifetimes with stable and reproducible retention

This chemistry is well established for the analysis of:

  • Polar and non‑polar peptides

  • Amino acids

  • Metabolites

  • Other challenging small molecules analyzed under HILIC conditions

Practical Operation

Despite its extended length and enhanced resolving power, the 70000‑15P‑2 delivers near‑UHPLC separation efficiency without requiring UHPLC‑rated pressures, making it a practical choice for laboratories seeking high resolution with existing instrumentation.

2.1 mm ID column system requirements:

  • Typical operating flow rates: ~0.2–0.3 mL/min

  • Requires an HPLC system capable of accurate, stable low‑flow delivery.

  • Not all standard HPLC pumps are suitable for reliable operation at these flow rates.

 

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