Objective:
To verify that the column oven maintains accurate and uniform temperature control across a defined range.

Materials Needed:

• Calibrated digital thermometer or NIST-traceable temperature probe (e.g., thermocouple or RTD)
• Insulated column blank or stainless steel tubing (to simulate a column)
• Data logging software (optional, for continuous monitoring)

Procedure:

1. Set Target Temperatures:

   • Choose at least three setpoints across the operating range (e.g., 25 °C, 40 °C, 60 °C).

2. Insert Probe:

   • Place the temperature probe inside the column oven, ideally inside a column blank to mimic actual conditions.

3. Stabilize:

   • Allow the oven to equilibrate for at least 30 minutes at each setpoint.

4. Record Temperature:

   • Measure and record the actual temperature at each setpoint.
   • Repeat measurements at multiple positions (if possible) to assess uniformity.

5. Evaluate Accuracy:

   • Compare measured values to setpoints.
   • Acceptable deviation is typically ±1 °C (check your lab’s SOP or regulatory guidelines).

6. Document Results:

   • Record all data, including date, instrument ID, probe calibration certificate, and environmental conditions.

Optional:

• Perform a time-based stability test by logging temperature over 1–2 hours to assess drift or fluctuation.