Streptomycin and Impurities Analyzed by HPLC - AppNote
November 19, 2012
/
/
/
/
Retention of a Highly Polar Antibiotic
Streptomycin is very polar and difficult to retain with Reversed Phase methods using conventional Columns. In this Method, retention is achieved with Separation of the Streptomycin from two impurities found in the Standard Solution. Furthermore, the numerous amine groups do not cause tailing of Peak shape as with standard silica-based Columns.
Data from two Column lots shown in the figure illustrates the reproducibility of the Method and it robustness.
Column: Cogent Diamond Hydride™, 4 µm, 100 A
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase:
Post Time: 3 minutes
Injection Vol.: 1 μL
Flow Rate: 1. mL / minute
Detection: UV @ 205 nm
Sample Preparation: 1.0 mg / mL Streptomycin Sulfate reference standard in Solvent A diluent.
t 0 : 0.9 minutes
Note: Streptomycin is an aminoglycoside antibiotic derived from the Actinobacterium Streptomyces Griseus. Streptomycin was isolated in 1943 and was the first antibiotic to treat tuberculosis.
Streptomycin is very polar and difficult to retain with Reversed Phase methods using conventional Columns. In this Method, retention is achieved with Separation of the Streptomycin from two impurities found in the Standard Solution. Furthermore, the numerous amine groups do not cause tailing of Peak shape as with standard silica-based Columns.
Data from two Column lots shown in the figure illustrates the reproducibility of the Method and it robustness.
Peaks: 1. Impurity, 2. Streptomycin, 3. Impurity
Method ConditionsColumn: Cogent Diamond Hydride™, 4 µm, 100 A
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase:
- A: DI Water / 0.1% Trifluoroacetic Acid (v/v)
- B: Acetonitrile/ 0.1% Trifluoroacetic Acid (v/v)
| Time (minutes) | %B |
|---|---|
| 0 | 95 |
| 1 | 95 |
| 6 | 40 |
| 7 | 95 |
Post Time: 3 minutes
Injection Vol.: 1 μL
Flow Rate: 1. mL / minute
Detection: UV @ 205 nm
Sample Preparation: 1.0 mg / mL Streptomycin Sulfate reference standard in Solvent A diluent.
t 0 : 0.9 minutes
Note: Streptomycin is an aminoglycoside antibiotic derived from the Actinobacterium Streptomyces Griseus. Streptomycin was isolated in 1943 and was the first antibiotic to treat tuberculosis.