Taurine Analyzed with LC-MS - AppNote
May 23, 2013
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Retention of a Polar, Sulfonate Compound
As a highly polar compound, Taurine is often difficult to retain by Reversed Phase Chromatography. The Peak Shape and Retention obtained with this HILIC LC-MS Method is greatly improved and is suitable as a starting point for further analyses of Taurine, in a variety of Samples such as beverages or even biological matrices.
Column: Cogent Diamond Hydride™, 4 μm, 100 Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150 mm
Mobile Phase:
Post Time: 3 minutes (3.3 column volumes)
Injection vol.: 2 μL
Flow rate: 0.4 mL / minute
Detection: ESI – POS - Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation: 10 mg / L of Taurine reference standard was added to a diluent of 50:50 Solvent A / Solvent B.
t 0 : 0.9 minutes
Note: Taurine is added to many popular energy drinks. It is found naturally in animal tissues and is a major constituent of bile.
Attachment No 251 Taurine Analyzed with LCMS pdf 0.3 Mb Download File
As a highly polar compound, Taurine is often difficult to retain by Reversed Phase Chromatography. The Peak Shape and Retention obtained with this HILIC LC-MS Method is greatly improved and is suitable as a starting point for further analyses of Taurine, in a variety of Samples such as beverages or even biological matrices.
Peak: Taurine, m/z 126.0219 [M+H]+
Method ConditionsColumn: Cogent Diamond Hydride™, 4 μm, 100 Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150 mm
Mobile Phase:
- A: DI Water with 0.1% Formic Acid (v/v)
- B: Acetonitrile with 0.1% Formic Acid (v/v)
| Time (minutes) | %B |
|---|---|
| 0 | 95 |
| 1 | 95 |
| 6 | 30 |
| 7 | 30 |
| 8 | 95 |
Post Time: 3 minutes (3.3 column volumes)
Injection vol.: 2 μL
Flow rate: 0.4 mL / minute
Detection: ESI – POS - Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation: 10 mg / L of Taurine reference standard was added to a diluent of 50:50 Solvent A / Solvent B.
t 0 : 0.9 minutes
Note: Taurine is added to many popular energy drinks. It is found naturally in animal tissues and is a major constituent of bile.
Attachment No 251 Taurine Analyzed with LCMS pdf 0.3 Mb Download File