MICROSOLV does not currently offer dedicated low-adsorption tubing that utilizes specialized surface treatments designed specifically for protein analysis.

However, for many low-pressure applications involving proteins, FEP tubing is generally the preferred option among the tubing materials we offer.

FEP vs. PTFE Tubing for Protein Applications

When selecting tubing for protein analysis, surface characteristics can influence sample recovery and analyte adsorption.

FEP Tubing

FEP tubing offers:

• Excellent chemical resistance
• Smooth, non-porous interior surfaces
• Low extractables
• Good visibility of fluids within the tubing
• Reduced potential for protein retention compared to PTFE

Because FEP does not contain the micro-porous structure associated with PTFE, it is often the preferred choice when minimizing protein interaction with tubing surfaces is important.

PTFE Tubing

PTFE tubing also provides excellent chemical compatibility and solvent resistance.

However:

• PTFE contains microscopic pores within its structure.
• Certain proteins may interact with or migrate into these pores.
• Protein recovery may be reduced in some applications.

For this reason, PTFE is typically considered less desirable than FEP when protein adsorption is a primary concern.

Application-Specific Testing Is Recommended

Protein behavior can vary significantly depending on:

• Protein size
• Molecular structure
• Concentration
• Buffer composition
• Mobile phase conditions
• Contact time
• Temperature

Because adsorption characteristics vary between proteins, suitability should always be verified using actual samples and method conditions.

Laboratories should perform recovery studies and method validation experiments to confirm acceptable performance for their specific application.

Dedicated Low-Adsorption Tubing

Some manufacturers offer tubing with proprietary low-adsorption surface treatments specifically designed for protein, peptide, and biomolecule analysis.

These products may incorporate:

• Specialized internal coatings
• Surface modification technologies
• Protein-resistant treatments

MICROSOLV does not currently offer these specialty-treated tubing products.

Recommended Guidance

For low-pressure protein applications:

• FEP tubing is generally preferred over PTFE tubing.
• Application-specific testing should always be performed.
• Recovery studies are recommended before routine use.
• Method suitability should be verified using actual sample matrices.

Because protein adsorption is highly dependent on the specific analyte and operating conditions, no tubing material can be universally recommended for every protein application.

Key Takeaways

• MICROSOLV does not offer specialized low-adsorption tubing with proprietary protein-resistant surface treatments.
• FEP tubing is generally preferred over PTFE tubing for many protein applications.
• FEP offers similar chemical resistance while avoiding the micro-porous characteristics of PTFE.
• Protein adsorption behavior varies depending on the sample and method conditions.
• Application-specific testing is recommended before implementation.
• Recovery studies should be performed to verify suitability for individual protein analyses.

For low-pressure tubing specifications, material options, product images, and ordering information, view Low-Pressure Tubing Specifications, Material Options, Product Images, and Ordering Information.