CUSTOM ITEM: HPLC Column, Bidentate C18, 4um, 3.0mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑10P‑3 Cogent Bidentate C18™ HPLC Column is a custom‑manufactured, mid‑bore analytical column designed to deliver higher resolving power than 50 mm formats while significantly reducing solvent consumption compared to standard 4.6 mm ID columns. Built on Cogent TYPE‑C™ silica hydride technology, this column offers unique selectivity, rapid equilibration, and exceptional chemical robustness.
The stationary phase uses an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry produces a phase that is highly hydrophobic yet versatile, capable of retaining compounds that often show limited or unstable retention on conventional silica‑based C18 columns. Unlike traditional C18 phases, this column can operate with 100 % aqueous mobile phases without phase collapse and tolerates very low pH conditions (below pH 2) without degradation.
The 3.0 mm internal diameter provides a practical balance between performance and efficiency—offering run times similar to 4.6 mm ID columns while using lower flow rates and much less mobile phase. No specialized low‑flow instrumentation is required, making this format easy to implement on standard analytical HPLC systems.
Why Choose the 3.0 × 100 mm Bidentate C18™ Format
This column is commonly selected when:
Additional resolution is required beyond 50 mm columns
Solvent‑use reduction is a priority without changing instrumentation
Methods benefit from the unique selectivity of silica hydride chemistry
Operation at low pH or with aqueous‑dominant mobile phases is required
The 100 mm length increases plate count and separation efficiency, while the 3.0 mm ID reduces solvent consumption—making this a strong choice for performance‑ and sustainability‑focused laboratories.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than conventional C18 columns
Extremely robust column lifetime
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Unique selectivity not achievable with standard C18 phases
Retains strongly hydrophobic and select hydrophilic analytes
Broad Mobile‑Phase Compatibility
Stable in 100 % aqueous mobile phases
Resistant to degradation at very low pH (below 2)
Suitable for aggressive or unconventional reversed‑phase methods
Solvent‑Efficient Analytical Geometry
3.0 mm ID × 100 mm length
Higher resolution than 50 mm columns
Lower solvent usage than 4.6 mm ID columns
Compatible with standard analytical HPLC systems
Typical Applications
Analytical HPLC requiring enhanced resolution
Method development and optimization
Compounds poorly retained on conventional C18 columns
Low‑pH separations
Aqueous‑dominant reversed‑phase methods
Solvent‑reduction initiatives
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable