Catalog Number - 40060-15P-2

The 40060‑15P‑2 Diol HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime compared to conventional bonded‑silica diol phases. The 4 µm silica hydride particles provide dependable chromatographic performance with moderate backpressure, making this column suitable for extended analytical runs and LC‑MS method development.

The TYPE‑C™ silica hydride surface ensures stable, reproducible retention across reversed‑phase (RP), normal‑phase (NP), and HILIC modes, supporting flexible and exploratory workflows.

Application Focus: Diol Chemistry for Polar Compounds

Diol stationary phases are widely used for polar analytes that are difficult to retain in reversed‑phase chromatography. The 40060‑15P‑2 column is especially effective for:

• Polar and non‑polar peptides

• Amino acids

• Polar metabolites

• Small biological and biomolecular compounds

Its balanced polarity allows reliable operation across RP, NP, and HILIC methods, making it a popular choice for new LC‑MS method development involving diverse polar compound classes.

Cogent TYPE‑C™ Silica Hydride Advantages

Relative to traditional bonded‑silica Diol columns, the TYPE‑C™ silica hydride platform provides tangible operational benefits:

• Faster equilibration, particularly valuable in HILIC workflows

• Excellent selectivity for polar and mixed‑polarity analytes

• Improved durability and extended column lifetime

• Reduced susceptibility to silica hydrolysis, enhancing robustness

These characteristics support consistent method performance during method scouting, optimization, and validation.

Why Choose the 2.1 mm × 150 mm Format

The 150 mm column length offers increased resolving power compared to shorter formats, while the 2.1 mm internal diameter maintains LC‑MS compatibility. This configuration is optimized for:

• Maximum resolution of complex polar mixtures

• Higher peak capacity for closely eluting analytes

• Improved separation confidence during method development

• Reduced solvent consumption relative to larger‑diameter columns

This format is well suited for metabolomics, peptide mapping, and exploratory LC‑MS workflows where resolution is prioritized over shortest run time.

Particle Size and Performance

The 4 µm particle size provides a balanced combination of efficiency, durability, and manageable backpressure, offering:

• Consistent peak shape and retention

• Broad compatibility with standard HPLC systems

• Reliable performance during longer chromatographic runs

This makes the column particularly appropriate for extended gradients common in method development.

LC‑MS‑Focused Operation

The narrow‑bore geometry supports LC‑MS and LC‑MS/MS workflows by:

• Delivering sharp, well‑defined peaks

• Reducing solvent load to the MS source

• Supporting stable and reproducible quantitative performance

These features contribute to its popularity for developing and refining new LC‑MS methods involving polar compounds.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery

• Important note: Not all standard HPLC systems are optimized for precise operation at these reduced flow rates

Flow rates should be optimized based on mobile‑phase composition, viscosity, column temperature, and system pressure limits.