HPLC Column, Diol, 4um, 4.6mm ID x 250mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40060‑25P Diol HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime compared with conventional bonded‑silica diol columns. The 4 µm silica hydride particles provide stable, reproducible chromatographic performance at moderate operating pressures, making this column particularly well suited for long analytical separations.
The TYPE‑C™ silica hydride surface supports reliable retention behavior across reversed‑phase (RP), normal‑phase (NP), and HILIC methods, enabling flexible method development with improved consistency and durability.
Application Focus: Diol Chemistry for Polar Compounds
Diol stationary phases are widely selected for the separation of polar compounds that show inadequate retention in reversed‑phase chromatography. The 40060‑25P column is effective for a broad range of analytes, including:
Polar and non‑polar peptides
Amino acids
Polar metabolites
Small biological and biomolecular compounds
Its balanced polarity allows operation across RP, NP, and HILIC modes, making it a very popular column for new method development when separation challenges require increased resolving power.
Cogent TYPE‑C™ Silica Hydride Advantages
Compared to traditional bonded‑silica Diol columns, the TYPE‑C™ silica hydride platform offers meaningful performance advantages:
Faster equilibration, especially beneficial for HILIC workflows
Excellent selectivity for polar and mixed‑polarity analytes
Improved robustness and extended column lifetime
Reduced susceptibility to silica hydrolysis, enhancing long‑term stability
These features support reproducible results during extended gradient runs and repeated method optimization cycles.
Why Choose the 4.6 mm × 250 mm Format
The 250 mm column length provides the highest resolving power within the Diol 4 µm analytical‑scale family. Combined with the 4.6 mm internal diameter, this format is optimized for:
Maximum resolution of complex polar mixtures
High peak capacity for closely eluting analytes
Improved separation confidence during method development and validation
Standard analytical HPLC compatibility without specialized hardware
This configuration is ideal when chromatographic resolution is prioritized over run time.
Particle Size and Performance
The 4 µm particle size provides a practical balance of efficiency and robustness, offering:
Stable peak shape over long gradients
Moderate pressure requirements
Broad compatibility with conventional HPLC systems
This makes the column well suited for extended analyses common in method development and optimization.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~0.8–1.2 mL/min
System compatibility: Designed for standard analytical HPLC instruments
Operational considerations: Flow rates should be adjusted based on mobile‑phase viscosity, temperature, and system pressure limits
No specialized low‑flow or UHPLC hardware is required.