HPLC Column, Diol 2.o, 2.2um, 2.1mm ID x 100mm Length, 120A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40260‑10P‑2 Diol 2.o™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, combining the proven selectivity of Diol chemistry with the performance advantages of a silica hydride surface. The 2.2 µm particle size delivers increased efficiency, excellent selectivity, and faster equilibration than conventional bonded‑silica diol phases, while remaining compatible with non‑UHPLC instruments under typical operating pressures.
Classified as “near‑UHPLC”, this column allows laboratories to achieve improved resolution and sharper peak shapes on standard HPLC systems without the need for UHPLC‑rated hardware.
Application Focus: Diol Chemistry for HILIC & Polar Compounds
Diol stationary phases are a standard choice for HILIC and normal‑phase chromatography due to their strong hydrophilicity and predictable hydrogen‑bonding behavior. The Diol 2.o™ phase is well suited for polar compounds that are difficult to retain in reversed‑phase methods, including:
Amino acids
Polar metabolites
Small biological and biomarker compounds
Other highly water‑soluble analytes
When operated in HILIC mode, this column is considered mass‑spectrometry friendly, supporting MS‑compatible mobile phases while delivering consistent retention and selectivity for polar analytes.
Cogent TYPE‑C™ Silica Hydride Advantages
Unlike traditional bonded‑silica diol columns, Diol 2.o™ is built on a silica hydride surface, providing operational advantages that improve method robustness:
Faster equilibration than conventional silica‑based diol phases
Improved efficiency and peak shape from 2.2 µm particles
Excellent selectivity for challenging polar compounds
Enhanced stability and reproducibility during gradient HILIC methods
The silica hydride surface reduces excessive dependence on unstable water layers, enabling more consistent chromatography and shorter re‑equilibration times between injections.
Why Choose the 2.1 mm × 100 mm Format
The 100 mm column length provides increased resolving power compared to shorter formats, while maintaining LC‑MS compatibility through the narrow 2.1 mm internal diameter. This configuration is optimized for:
Higher resolution separations of complex polar mixtures
Improved peak capacity for closely eluting analytes
Balanced run times with enhanced separation confidence
Efficient solvent use compared to larger‑diameter columns
This format is well suited for applications where resolution is prioritized over maximum speed, including metabolomics and detailed biomarker analysis.
Particle Size and Separation Efficiency
The 2.2 µm particle size delivers near‑UHPLC performance, producing narrow, symmetrical peaks and improved resolving power while typically remaining within the pressure limits of many standard HPLC systems under common operating conditions.
LC‑MS‑Optimized Performance
The combination of narrow internal diameter and stable Diol retention mechanism supports:
Improved signal‑to‑noise ratios
Reliable, reproducible peak shapes
Consistent quantitative performance
These attributes make the 40260‑10P‑2 column a strong choice for LC‑MS and LC‑MS/MS analysis of polar and semi‑polar compounds.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery
Important note: Not all standard HPLC pumps or flow cells are suitable for precise operation at reduced flow rates
Final flow rates should be optimized based on mobile‑phase composition, viscosity, column temperature, and system pressure limits.