High‑Purity Phenyl Columns with Excellent Peak Shape
Double End‑Capped | Unique Selectivity | Reversed‑Phase HPLC
Cogent HPS™ Phenyl HPLC columns are engineered for high efficiency, excellent peak shape, and reliable selectivity in reversed‑phase (RP) HPLC. These columns are manufactured using proprietary, high‑purity silica and are designed to deliver consistent performance for both in‑house and USP methods.
With decades of proven laboratory use, Cogent HPS™ Phenyl remains a trusted choice for analysts who require clean separations of aromatic compounds and peptides.
Advanced Phenyl Chemistry on High‑Purity Silica
Cogent HPS™ Phenyl columns are base‑deactivated and mono‑functional bonded, helping minimize secondary interactions that can lead to peak tailing. The stationary phase is exhaustively double end‑capped, further reducing residual silanol activity and improving peak symmetry.
This carefully controlled surface chemistry results in:
Improved peak shape
Enhanced resolution
Consistent retention behavior
Reliable performance across routine RP methods
Designed for Reversed‑Phase HPLC
Cogent HPS™ Phenyl columns are packed and ready for reversed‑phase HPLC, offering aromatic selectivity through π–π interactions while maintaining robust hydrophobic retention. These characteristics make them especially useful for analytes that do not separate well on C18 columns.
For more than 20 years, laboratories have successfully used Cogent HPS™ Phenyl columns for routine QC, research, and USP‑based methods, making them a proven and dependable phenyl phase.
Ideal for Aromatic Compounds and Peptides
Cogent HPS™ Phenyl columns are particularly well suited for:
Aromatic and polyaromatic compounds
Pharmaceutical intermediates and impurities
Peptide analysis
Structurally rigid or conjugated molecules
The balanced selectivity of the phenyl phase provides reliable alternatives when alkyl phases lack sufficient resolution.
Available Formats and Configurations
Cogent HPS™ Phenyl columns are available to support both analytical and scale‑up workflows:
Analytical columns
Guard columns
Preparative columns
5 µm particle size
120 Å pore size
This flexibility allows method transfer from analytical to preparative HPLC without changing selectivity.
Key Features and Benefits
Manufactured with high‑purity silica
Base‑deactivated surface chemistry
Mono‑functional bonding
Exhaustive double end‑capping
Excellent peak shape and resolution
Optimized for reversed‑phase HPLC
Proven performance in USP and legacy methods
Suitable for aromatic compounds and peptides
Available in analytical, guard, and prep formats
Typical Applications
Aromatic pharmaceuticals
Polyaromatic compounds
Peptide separations
USP® and legacy reversed‑phase methods
Research, QC, and GMP laboratories
Additional Resources
Column Protection Recommendation
Important: MICROSOLV technical support strongly recommends using the Cogent Column Filter, which is included with each column, whenever possible. Column filters help protect against particulates, extend column lifetime, and maintain consistent chromatographic performance.
👉 Learn more about the Cogent Column Filter for HPLC column protection
