How to calibrate UV-vis and HPLC detectors with NIST traceable solutions below 230nm - Tips & Suggestions
April 10, 2012
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Date: 10-APRIL-2012 Last Updated: 5-JULY-2025
Holmium Oxide is an internationally recognized wavelength calibration primary standard, supported by NIST and other standards organizations.
The Chemical Solutions Qualification Kits™ have been designed so that the combination of Caffeine with Holmium Oxide will provide NIST-Traceable wavelength calibration down to 205 nm. First using Holmium Oxide, the detector is calibrated as described in the instructions, at 241 nm and above. Next, the wavelengths of Caffeine are determined at 205 nm and 273 nm. NIST traceability is achieved by the 273 nm band, which overlaps the official NIST range.
Assuming that the detector meets at this wavelength, the 205 nm band becomes NIST traceable, and can be used with confidence to ascertain the Wavelength Accuracy of the detector at this lower Wavelength.
Using these two standards in combination with each other, provides a means to Qualify your detector from 205 nm to 641 nm with full confidence.