Fexofenadine Analyzed with HPLC - AppNote
May 16, 2012
/
/
/
Date: 16-MAY-2012 Last Updated: 10-AUGUST-2026
Simple Isocratic Assay Method without Amine AdditivesHPLC Column Specifications, Applications, Configurations, and Ordering Information
With this Method, Trifluoroacetic Acid is used instead and good Peak shapes are observed, as the five Chromatograms that are overlaid in the Figure show.
Peak: Fexofenadine
Method ConditionsColumn: Cogent Phenyl Hydride™, 4 μm, 100 Å
Catalog No.: 69020-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase: 62% DI Water / 38% Acetonitrile / 0.1% Trifluoroacetic Acid (TFA)
Temperature: 35˚C
Flow rate: 1.0 mL / minute
Detection: UV @ 220 nm
Injection vol.: 1 μL
Sample Preparation:
- Stock Solution: 180 mg strength Allegra® (Fexofenadine) tablet was ground and added to a 100 mL volumetric flask. It was diluted with the Mobile Phase and vortexed 5 minutes. A portion was filtered with a 0.45 µm Nylon Syringe Filter (MICROSOLV Tech Corp.).
- Working Solution: A 100 µL aliquot of the Stock Solution was diluted with 900 µL of the Mobile Phase.
Note: Fexofenadine is marketed under the trade name Allegra, but generic versions are also available. In January 2011, the Food and Drug Administration approved over-the-counter sales of the drug without a prescription. It is a widely selling antihistamine used for treatment of hay fever and other allergies. Because it does not cross the blood-brain barrier, it causes less drowsiness than first generation antihistamines.
Attachment No 139 Fexofenadine Analyzed with HPLC pdf 0.3 Mb Download File