PAH Polycyclic Aromatic Hydrocarbons Analyzed with HPLC - AppNote
May 22, 2012
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Analysis of PAH by Reversed Phase HPLC
Polycyclic Aromatic Hydrocarbons (PAH) determination in soil, food, air, body fluids etc. has been a topic of interest for routine Quality Control or Screening Analyses. This Method offers good Resolution, reproducible Retention Time and Peak Shape for these compounds. In addition to the main components, small peaks due to impurities or decomposition products are well Resolved in the easy, isocratic Method.
Column: Cogent Bidentate C18™, 4 μm, 100 Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase: 70:30 Acetonitrile / DI Water
Injection vol.: 1 μL
Flow rate: 0.5 mL / minute
Detection: UV @ 25 4nm
Sample Preparation: 1 mg of each sample was dissolved in 1 mL of the Mobile Phase.
Note: A Polycyclic Aromatic Hydrocarbon is a Hydrocarbon Compound containing only Carbon and Hydrogen—that is composed of multiple Aromatic Rings.
Attachment No 29 Polycyclic Aromatic Hydrocarbons Analyzed with HPLC pdf 0.2 Mb
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Polycyclic Aromatic Hydrocarbons (PAH) determination in soil, food, air, body fluids etc. has been a topic of interest for routine Quality Control or Screening Analyses. This Method offers good Resolution, reproducible Retention Time and Peak Shape for these compounds. In addition to the main components, small peaks due to impurities or decomposition products are well Resolved in the easy, isocratic Method.
Peaks:
1. Naphthalene,
2. Phenanthrene,
3. Guaifenesin,
4. Pyrene
I. Impurities or Decomposition Product
Column: Cogent Bidentate C18™, 4 μm, 100 Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase: 70:30 Acetonitrile / DI Water
Injection vol.: 1 μL
Flow rate: 0.5 mL / minute
Detection: UV @ 25 4nm
Sample Preparation: 1 mg of each sample was dissolved in 1 mL of the Mobile Phase.
Note: A Polycyclic Aromatic Hydrocarbon is a Hydrocarbon Compound containing only Carbon and Hydrogen—that is composed of multiple Aromatic Rings.
Attachment No 29 Polycyclic Aromatic Hydrocarbons Analyzed with HPLC pdf 0.2 Mb
Download File