Troubleshooting: No Peak Detected

If you do not see a peak during analysis, follow these steps to investigate possible reasons:

1. Check Column Connection

   • Detach the column and replace it with a column union.
   • Inject the solution again.
     • If a peak appears: The issue may be with your column (e.g., poor retention or elution).
     • If no peak appears: Continue to the next steps.

2. Verify Sample Preparation

   • Ensure the analyte is properly extracted and present in the sample.
   • Confirm the sample level is sufficient for the autosampler syringe.
     • If the level is too low, the needle may aspirate air instead of the sample.

3. Inspect Detector Performance

   • A malfunctioning detector can prevent peak detection. Check for proper operation.

4. Confirm Flow Path

   • On systems with multiple column options, verify that flow is directed to your column.
   • If there is no pressure with the column connected, flow may not be reaching it.