Ketorolac Separation with LCMS - AppNote
December 12, 2016
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Date: 12-DECEMBER-2016 Last Update: 14-AUGUST-2026
Analgesic Agent Analyzed in Tablet FormCogent™ Phenyl Hydride HPLC Column Catalog Numbers, Column Specifications, Available Dimensions, and Ordering Information
The chromatograms show how the compound can be retained and detected using either UV-based detection (Fig. A) or Mass Spectrometry (Fig. B). The conditions may be applied to the routine assay analyses of Ketorolac Tablet formulations.
Peak:
Ketorolac
256.2 m/z [M+H]+
Column: Cogent Phenyl Hydride™, 4 µm, 1 00Å
Catalog No.: 69020-10P-2
Dimensions: 2.1 x 100 mm
Solvents:
-- A: DI Water/ 0.1% Formic Acid (v/v)
-- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 20 |
| 5 | 80 |
| 10 | 80 |
| 11 | 20 |
Injection vol.: 1 µL
Flow rate: 0.5 mL / minute
Detection:
-- Fig. A: UV 320 nm
-- Fig. B: ESI-POS- Mass Spec
Sample: Ground Ketorolac Tablet was extracted with DI Water (1 mg / mL powder concentration) and gravity filtered. Sample was then filtered through a 0.45 µm Syringe filter for HPLC analysis.
Ketorolac by HPLC and LCMS pdf 0.2 Mb Download File