Improved Peak Shape of Tetracycline Using a Dursan Coated Steel HPLC Column - AppNote
September 28, 2022
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Improved peak shape of a tetracycline antibiotic
Met hod Conditions:
Oxytetracycline has a role as an antibacterial drug, a protein synthesis inhibitor, an antimicrobial agent, an anti-inflammatory drug, and a bacterial metabolite. However, this compound interacts with stainless steel during HPLC and is known to have substandard peak shapes.
This data highlights important chromatographic performance improvements between our metal free treated stainless steel HPLC column and untreated stainless steel column with the same packing material and method conditions on the same instrument. Metal free surface treatments are available on all Cogent HPLC columns upon request.
Peak:
Oxytetracycline
| Trace | Hardware | Width (Minutes) | Plates (N) |
| Red | Metal Free Coated Steel | 0.46 | 304 |
| Blue | non Coated Stainless Steel | 1.18 | 43 |
Met hod Conditions:
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15D-2 and, 70000-15P-2
Dimensions of both columns: 2.1mm x 150mm
Mobile Phase: 50% acetonitrile / 50% DI water / 0.1% formic acid
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: 270nm
Sample Preparation: o xytetracycline 0.2 mg / mL in DI water
Catalog No.: 70000-15D-2 and, 70000-15P-2
Dimensions of both columns: 2.1mm x 150mm
Mobile Phase: 50% acetonitrile / 50% DI water / 0.1% formic acid
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: 270nm
Sample Preparation: o xytetracycline 0.2 mg / mL in DI water
Note:
Oxytetracycline is known as a broad-spectrum antibiotic due to its activity against such a wide range of infections. Oxytetracycline inhibits cell growth by inhibiting translation. It binds to the 30S ribosomal subunit and prevents the amino-acyl tRNA from binding to the A site of the ribosome.