Phenolic Compounds found in Rice Extract Analyzed with LCMS - AppNote
January 26, 2014
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Date: 21-JANUARY-2014 Last Update: 15-AUGUST-2026
Content of Phenolic Compounds in Rice Determined by LC-MSWhen commercial Rice Extracts were analyzed, only 6’-O-Feruloylsucrose at 3.01 minutes (Peak not shown for the clarity of the Chromatogram) was found in this particular Rice Extract Sample. Next, the Sample was spiked with the Standards according to the literature referenced below [1] .
This Method is an excellent choice to use for this analysis of these Phenolic Compounds.
Peaks:
1. Gallic Acid
169 m/z [M-H]–
2. Caffeic Acid
179 m/z [M-H]–
3. p-Coumaric Acid
163 m/z [M-H]–
4. Ferulic Acid
193 m/z [M-H]–
5. 3,5-Dimethoxy-4-Hydroxycinnamic Acid
223 m/z [M-H]–
Column: Cogent Phenyl Hydride™, 4 μm, 100 Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50 mm
Mobile Phase:
- A: DI Water / 0.1% Formic Acid (v/v)
- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 10 |
| 5 | 20 |
| 6 | 20 |
| 7 | 10 |
Post Time: 3 minutes
Injection vol.: 1 μL
Flow rate: 0.4 mL / minute
Detection: ESI – NEG - Perkin Elmer, Flexar SQ 300 Mass Spectrometer
Sample Preparation: Commercial Rice Extract was spiked with standards at a concentration of 12.5 ppm and was analyzed.
Note: Rice is a staple food in many countries. It contains phenolic compounds which have anticancer, antioxidant, and anti-mutagenic effects. It is important to analyze Rice Extracts to confirm the content of the phenolic compounds in rice.
[1] J.E. Hayes, P. Allen, N. Brunton, M.N. O’Grady, and J.P. Kerry, Food Chemistry, 126, (2011) 948–955
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