Overview
The U-2D™ Micro-Sample Management System is designed for low-volume sample storage, preparation, and analysis in HPLC, UHPLC, and LC-MS workflows. The system uses precision glass inserts within an ANSI/SLAS-compatible sample management format, enabling efficient handling of microliter-scale sample volumes.
When U-2D™ inserts are used in regulated or controlled laboratory environments, sterilization and decontamination procedures may be required before use.
Insert Construction
U-2D™ inserts are manufactured from high-quality Type 33 borosilicate glass, a material commonly used throughout analytical laboratories because of its excellent chemical resistance and thermal stability.
Key features include:
- Precision-manufactured glass construction
- Low extractable characteristics
- Excellent chemical compatibility
- Suitability for analytical applications
- Design optimized for low-volume sample handling
The insert geometry is engineered to support efficient sample recovery and reliable sample management.
Compatibility with Wide-Opening Autosampler Vials
When used with compatible wide-opening 2 mL screw-top autosampler vials, U-2D™ inserts are self-aligning within the vial.
Benefits include:
- Simplified assembly
- Reliable positioning
- Elimination of support springs
- Reduced component count
- Improved workflow efficiency
This design helps streamline sample preparation and analytical workflows.
Silicone Mat Compatibility
The silicone sealing mat used with the U-2D™ system is suitable for HPLC and LC-MS applications.
The mat may be handled similarly to other laboratory-grade silicone sealing systems and is designed to provide effective well coverage during sample storage and processing.
Sterilization of U-2D™ Inserts
Autoclave Sterilization
U-2D™ glass inserts may be sterilized using validated autoclave procedures appropriate for Type 33 borosilicate laboratory glassware.
Autoclaving may be performed using:
- Approved laboratory steam cycles
- Site-specific validation protocols
- Established drying procedures
Laboratories should follow their internal sterilization requirements and qualification procedures.
Alternative Sterilization Methods
When required by laboratory SOPs, alternative sterilization methods may also be considered.
Examples may include:
- Gas sterilization processes
- Chemical sterilization procedures
- Other validated laboratory sterilization techniques
As with any analytical glassware, care should be taken to prevent residue formation, contamination, or surface damage.
Important Note Regarding Plastic Components
The U-2D™ system contains both glass and plastic components.
Glass inserts and plastic rack assemblies may have different temperature limitations and sterilization requirements.
Before sterilizing non-glass components:
- Review the applicable U-2D™ instructions.
- Follow component-specific processing recommendations.
- Confirm compatibility with the selected sterilization method.
Not all system components should be assumed to have identical sterilization capabilities.
Centrifugation Compatibility
U-2D™ inserts may be processed using centrifugation procedures commonly applied to borosilicate laboratory consumables.
Users should always:
- Follow rotor manufacturer specifications.
- Follow instrument operating limits.
- Verify assembly compatibility.
- Follow laboratory validation requirements.
Centrifugation parameters should be established according to laboratory procedures and equipment capabilities.
Storage Recommendations
U-2D™ inserts may be stored using practices commonly applied to analytical laboratory glassware.
Recommended practices include:
- Storage in clean environments
- Protection from physical damage
- Protection from contamination
- Inspection before use
- Proper handling during transport and processing
Good laboratory practices should be followed at all times.
Best Practices
Pre-Use Cleaning
Prior to use, laboratories may wish to:
- Rinse inserts using appropriate solvent grades.
- Follow internal cleaning procedures.
- Document cleaning activities when required.
Sterilization Inspection
Following sterilization:
- Inspect inserts for chips or cracks.
- Verify cleanliness.
- Examine for visible residues.
- Remove damaged inserts from service.
Sample Integrity
For critical analytical applications:
- Minimize unnecessary handling.
- Avoid contamination during transfer.
- Verify system cleanliness before use.
Silicone Mat Management
For highly sensitive LC-MS applications:
- Limit unnecessary punctures.
- Replace mats when appropriate.
- Monitor for signs of wear.
Key Takeaways
- U-2D™ inserts are manufactured from Type 33 borosilicate glass.
- Inserts may be sterilized using validated procedures commonly applied to laboratory borosilicate glassware.
- Glass inserts and plastic components may have different processing requirements.
- Inserts are compatible with many low-volume analytical workflows.
- Routine inspection helps maintain sample integrity and system performance.
- Proper handling, sterilization, and storage support reliable analytical results.