Overview

The U-2D™ Micro-Sample Management System was developed to integrate with standard 96-well laboratory equipment used for sample preparation, transfer, filtration, extraction, and automation. Its ANSI/SLAS-compliant footprint allows compatibility with many manually operated and automated laboratory systems designed around standard microplate dimensions.

This standardized format simplifies implementation within existing workflows while supporting efficient sample handling and transfer operations.

ANSI/SLAS Standard Compatibility

The U-2D™ system conforms to established ANSI/SLAS microplate standards for:

• Footprint dimensions
• Well spacing
• Deck positioning
• Automation compatibility

Because of this standardized design, the system can often be incorporated into equipment configured for traditional 96-well microplates without requiring custom modifications.

Assembly Dimensions

When used with the U-2D™ Base and Rack assembly:

• 350 µL and 500 µL Formats Approximate overall height: 1.625 inches
• 1 mL Format Approximate overall height: 2.0 inches

Before implementation, verify that:

• Manifold lids clear the assembly
• Vacuum gaskets fit correctly
• Pressure seals operate properly
• Automation deck heights are sufficient

SPE and Filtration Integration

Gravity-Based Processing

When gravity flow is used, the Rack may be operated independently without the Base.  This configuration is suitable when a sealed pressure or vacuum environment is not required.

Vacuum and Pressure Manifolds

For positive-pressure or vacuum-assisted processing:

• Use the Rack together with the U-2D™ Base.
• Verify gasket alignment before operation.
• Confirm seal integrity across the entire plate footprint.
• Adjust seal adapters as required by the manifold design.

Proper sealing helps maintain uniform flow across all sample positions.

Mechanical Load Considerations

The U-2D™ design includes molded recesses that allow additional racks to nest during storage or handling.

However:

• SPE plates should not rest directly on the sample rack.
• Filtration plates should not apply mechanical force to the U-2D™ assembly.
• Vertical loads should be minimized whenever possible.

Excessive pressure transfer may affect performance and could lead to component damage.

Maintaining slight clearance between stacked devices is recommended.

Centrifugation Guidance

For centrifuge applications:

• The Base must remain attached to the Rack.
• Validate rotor compatibility before use.
• Confirm proper balance procedures.

The U-2D™ assembly has been validated for use up to:  2,500 × g

If SPE or filtration devices are placed above the system during centrifugation, users should carefully evaluate clearances and loading conditions before operation.

Vortex Mixing Considerations

For routine vortex mixing:

Recommended maximum speed:

Approximately 3,000 RPM

Operating above this level may increase stress on:

• Inserts
• Gasket interfaces
• Rack components
• Sample holders

Following recommended speed limits helps maintain assembly integrity.

Automation Compatibility

Because the U-2D™ system follows ANSI/SLAS dimensional standards, many automation platforms can accommodate the assembly.

Before running production samples, verify:

• Deck clearances
• Gripper compatibility
• Z-axis travel limits
• Plate positioning accuracy
• Manifold alignment

Pilot testing is recommended before full-scale implementation.

Recommended Validation Checklist

Before routine use:

1. Verify assembly height compatibility.
2. Confirm manifold sealing performance.
3. Check that upper devices do not contact the rack.
4. Validate centrifugation conditions.
5. Verify vortex mixing settings.
6. Run water or surrogate samples to confirm flow performance.
7. Confirm automation clearances if robotic systems are used.

These checks can help ensure reliable operation and consistent sample processing.

Key Takeaways

• The U-2D™ system is compatible with ANSI/SLAS-standard 96-well platforms.
• It can be used with many SPE manifolds, filtration systems, and automation platforms.
• Rack and Base assemblies should be used together for vacuum, pressure, and centrifugation applications.
• Maximum validated centrifugation is 2,500 × g.
• Recommended vortex speeds should remain at or below approximately 3,000 RPM.
• Mechanical loads from stacked plates should be avoided.
• Validation of clearance and sealing performance is recommended before routine operation.

• For U-2D™ Micro-Sample Management System Dimensions, Accessories, Configurations, and Ordering Information, view U-2D™ Micro-Sample Management System.