Peptides, Lipophilic Non Polar Analyzed with HPLC - AppNote
April 30, 2021
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Simple Gradient for Non-Polar Peptides
In this Method, we demonstrate the Retention of several Hydrophobic Peptides without the use of a typical Reversed Phase Column.
Column: Cogent Diamond Hydride™, 4 μm, 100 Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150 mm
Mobile Phase:
Gradient:
Post time: 2 minutes
Injection vol.: 1 µL
Flow rate: 1.0mL / minute
Detection: UV @ 220 nm
Sample Preparation: 0.5 mg of each compound in 1 mL of Methanol. Sample for injection was diluted to 0.1 mg / mL with Acetonitrile.
t 0 : 0.95 minutes
Note: Biomolecules are a diverse range of compounds, amino acids, proteins, peptides, nucleic acids, and obtaining quality separations is of great importance. Gly-Tyr is a dipeptide which has a role as a metabolite. Enkephalins are a family of peptides which are present in the brain and are involved in the control of pain sensation. They are endogenous peptides that act as an antagonists at opioid receptors. Angiotensin increases blood pressure by causing narrowing of the blood vessels.
In this Method, we demonstrate the Retention of several Hydrophobic Peptides without the use of a typical Reversed Phase Column.
Peaks:
1. Gly-Tyr ( Glycyl-L-tyrosine ), 2. Val-Tyr-Val ( Valyltyrosylvaline ), 3. Metenkephalin ( Metenkefalin ), 4. Leuenkephalin, 5. Angiotensin II
Column: Cogent Diamond Hydride™, 4 μm, 100 Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150 mm
Mobile Phase:
- A: DI Water with 0.05% Trifluoroacetic Acid (TFA)
- B: Acetonitrile with 0.05% Trifluoroacetic Acid (TFA)
Gradient:
| Time ( minutes ) | %B |
|---|---|
| 0 | 10 |
| 1 | 10 |
| 15 | 30 |
| 16 | 10 |
Post time: 2 minutes
Injection vol.: 1 µL
Flow rate: 1.0mL / minute
Detection: UV @ 220 nm
Sample Preparation: 0.5 mg of each compound in 1 mL of Methanol. Sample for injection was diluted to 0.1 mg / mL with Acetonitrile.
t 0 : 0.95 minutes
Note: Biomolecules are a diverse range of compounds, amino acids, proteins, peptides, nucleic acids, and obtaining quality separations is of great importance. Gly-Tyr is a dipeptide which has a role as a metabolite. Enkephalins are a family of peptides which are present in the brain and are involved in the control of pain sensation. They are endogenous peptides that act as an antagonists at opioid receptors. Angiotensin increases blood pressure by causing narrowing of the blood vessels.