For LC‑MS and HPLC Analysis of Extremely Polar Compounds
Diamond Hydride™ HPLC columns are MICROSOLV’s best‑selling and highest‑performing column, widely regarded as the gold standard for LC‑MS and HPLC analysis of very polar compounds. These columns are especially effective for ANP / HILIC style separations, combining strong polar retention with controlled hydrophobicity.
Diamond Hydride™ columns are the most popular choice for scientists working in LC‑MS, metabolomics, bioanalytical research, and the analysis of amino acids, organic acids, carbohydrates, and other highly polar small molecules.
Unique Silica Hydride Surface Chemistry (TYPE‑C™)
Diamond Hydride™ columns are based on a silica hydride surface, similar to other TYPE‑C™ phases, but with a critical enhancement that sets them apart. A very small, controlled amount of carbon is incorporated on the surface, precisely tuning the hydrophobicity of the stationary phase.
This unique surface chemistry provides:
Strong retention of extremely polar compounds
Controlled hydrophobic interactions
Improved peak shape and reproducibility
Enhanced performance in ANP / HILIC like methods
Exceptional compatibility with LC‑MS
The result is a versatile column that bridges polar and moderately non‑polar analytes more effectively than traditional silica‑based phases.
Ideal for Complex and Biological Matrices
Diamond Hydride™ columns allow precise, robust methods to be developed even when working with complex sample matrices, including biological fluids such as:
Plasma
Urine
Saliva
Other bio‑matrices
Both polar and some non‑polar peptides can often be separated in the same run, making Diamond Hydride™ a powerful tool for comprehensive profiling and untargeted analysis.
Applications Where Diamond Hydride™ Excels
LC‑MS and LC‑MS/MS
Metabolomics and lipid‑polar hybrid studies
Bioanalytical and clinical research
Amino acids and organic acids
Carbohydrates and sugars
Very polar small molecules
Mixed polar / non‑polar analyte separations
Key Features and Benefits
Best‑selling and best‑performing MICROSOLV column
Designed for extremely polar compounds
Optimized for LC‑MS and HPLC
Unique silica hydride TYPE‑C™ surface
Precisely tuned hydrophobicity
Excellent performance in ANP / HILIC methods
Enables separation of polar and some non‑polar analytes in one run
Reliable performance with complex biological samples
Learn More and Technical Resources
Column Protection Recommendation
Important: MICROSOLV technical support strongly recommends using the Cogent Column Filter, which is included with each column, whenever possible. Column filters help protect the column from particulates, extend column lifetime, and maintain consistent chromatographic performance.
👉Learn more about the Cogent Column Filter for HPLC column protection
