Nonylphenol Analyzed by HPLC - AppNote
September 18, 2013
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Separation of Isomer Peaks using Normal Phase HPLC
Nonylphenol is a very hydrophobic compound and is suitable for analysis by Normal Phase. It is produced commercially by Acid-catalyzed Alkylation of Phenol with a mixture of nonenes. Therefore a variety of product isomers are possible, with different branching of the C9 group and position of the chain on the ring.
Normal Phase HPLC is well-suited to isomer separations and in this method, separation was observed between the main peak and two smaller peaks. Three runs are shown to illustrate the repeatability, which is often a concern with Normal Phase Methods that use ordinary Silica Columns.
Column: Cogent Silica-C™, 4 µm, 100 Å
Catalog No.: 40000-10P
Dimensions: 4.6 x 100 mm
Mobile Phase:
-- A: Ethyl Acetate
-- B: Hexane
Gradient:
Post Time: 3 minutes
Injection vol.: 1 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 277 nm
Sample Preparation: Nonylphenol reference standard dissolved in a Hexane diluent.
t 0 : 1.3 minutes
Note: Nonylphenols are used in synthesis as a starting material for various surfactants. They are subjected to Ethoxylation to produce Alkyl-Phenol Ethoxylates.
Attachment No 264 Nonylphenol HPLC Method pdf 0.3 Mb Download File
Nonylphenol is a very hydrophobic compound and is suitable for analysis by Normal Phase. It is produced commercially by Acid-catalyzed Alkylation of Phenol with a mixture of nonenes. Therefore a variety of product isomers are possible, with different branching of the C9 group and position of the chain on the ring.
Normal Phase HPLC is well-suited to isomer separations and in this method, separation was observed between the main peak and two smaller peaks. Three runs are shown to illustrate the repeatability, which is often a concern with Normal Phase Methods that use ordinary Silica Columns.
Main Peak: Nonylphenol
Method ConditionsColumn: Cogent Silica-C™, 4 µm, 100 Å
Catalog No.: 40000-10P
Dimensions: 4.6 x 100 mm
Mobile Phase:
-- A: Ethyl Acetate
-- B: Hexane
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 100 |
| 4 | 100 |
| 19 | 90 |
| 20 | 100 |
Post Time: 3 minutes
Injection vol.: 1 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 277 nm
Sample Preparation: Nonylphenol reference standard dissolved in a Hexane diluent.
t 0 : 1.3 minutes
Note: Nonylphenols are used in synthesis as a starting material for various surfactants. They are subjected to Ethoxylation to produce Alkyl-Phenol Ethoxylates.
Attachment No 264 Nonylphenol HPLC Method pdf 0.3 Mb Download File