Silica WCX columns | USP® L9 class | Ionized at pH 2.5 | Use to retain bases.
Ion exchange chromatography is the most popular method for the purification and analysis of charged molecules. (IEX) HPLC allows the separation of polar molecules (charged) including but not limited to amino acids, organic acids and bases, proteins or peptides, based on their affinity to ionic groups bonded on silica. The retention on the column is reversible by "exchange" of ions between the target ions in the mobile phase to the ions present on the ligands.
Cation Exchange columns have a negative charged ionic group with an affinity for polar molecules having a positive charge. These Cogent IEX WCX™ (weak cation exchange) HPLC columns have carboxylic acid functional groups bonded to the Cogent RP™ 5 or 10um, 100A silica. pH range 2.5-7.
"Weak" Ion Exchange is not related to the strength of ion retention or binding, but indicates that ionization occurs at low mobile phase pH and is not ionized at higher pH.
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